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Sökning: onr:"swepub:oai:DiVA.org:lnu-123543" > Surface modulation ...

  • Huang, TianweiUniv Tokyo, Sch Engn, Dept Bioengn, 7-3-1 Hongo,Bunkyo Ku, Tokyo 1138656, Japan. (författare)

Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells

  • Artikel/kapitelEngelska2023

Förlag, utgivningsår, omfång ...

  • Elsevier,2023
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:lnu-123543
  • https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-123543URI
  • https://doi.org/10.1016/j.reth.2022.12.007DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-507548URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Exosomes (diameter 30-200 nm) are a subtype of extracellular vesicles secreted by cells containing DNA, microRNA (miRNA), and proteins. Exosomes are expected to be valuable as a means of delivering drugs or functional miRNAs in treatment of diseases. However, the delivery of exosomes is not sufficiently effective, even though exosomes have intrinsic delivery functions. Cell-penetrating peptides (CPPs) are short peptide families that facilitate cellular intake of molecules and vesicles. We previously reported that the modification of cells, and liposomes with CPP-conjugated-lipids, CPPs conjugated with poly (ethylene glycol)-conjugated phospholipids (PEG-lipid), that induce adhesion by CPPs, can be useful for cell-based assays and harvesting liposomes. In this study, we aimed to modulate the exosome surface using Tat peptide (YGRKKRRQRRR)-PEG-lipids to improve intracellular delivery to endothelial cells. We isolated and characterized exosomes from the medium of HEK 293 T cell cultures. Tat conjugated PEG -lipids with different spacer molecular weights and lipid types were incorporated into exosomes using fluorescein isothiocyanate labeling to optimize the number of Tat-PEG-lipids immobilized on the exo-some surface. The exosomes modified with Tat-PEG-lipids were incubated with human umbilical vein endothelial cells (HUVECs) to study the interaction. Tat conjugated with 5 kDa PEG and C16 lipids incorporated on the exosome surface were highly detected inside HUVECs by flow cytometry. Fluores-cence was negligible in HUVECs for control groups. Thus, Tat-PEG-lipids can be modified on the exosome surface, improving the intracellular delivery of exosomes.(c) 2022, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Sato, YuyaUniv Tokyo, Sch Engn, Dept Bioengn, 7-3-1 Hongo,Bunkyo Ku, Tokyo 1138656, Japan. (författare)
  • Kuramochi, AkikoNatl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan. (författare)
  • Ohba, YoshioNatl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan. (författare)
  • Sano, MasayukiNatl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan. (författare)
  • Miyagishi, MakotoNatl Inst Adv Ind Sci & Technol, Biomed Res Inst, AIST Tsukuba Cent 6,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan. (författare)
  • Tateno, HiroakiNatl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan. (författare)
  • Wadhwa, RenuNatl Inst Adv Ind Sci & Technol, AIST INDIA DAILAB, Cent 5-41, Tsukuba 3058565, Japan.;Univ Tsukuba, Sch Integrat & Global Majors SIGMA, Tsukuba Life Sci Innovat, Tsukuba 3058577, Japan. (författare)
  • Kawasaki, KazunoriNatl Inst Adv Ind Sci & Technol, Res Inst Electrochem Energy, Dept Energy & Environm, Mat Sci RG, 1-8-31 Midorigaoka, Ikeda, Osaka 5638577, Japan. (författare)
  • Uchida, TakeyukiNatl Inst Adv Ind Sci & Technol, Res Inst Electrochem Energy, Dept Energy & Environm, Mat Sci RG, 1-8-31 Midorigaoka, Ikeda, Osaka 5638577, Japan. (författare)
  • Nilsson Ekdahl, KristinaUppsala universitet,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Uppsala University, Sweden,Linnaeus Ctr Biomat Chem, BMC,Vaskulärbiologi,Linnaeus Univ, Linnaeus Ctr Biomat Chem, SE-39182 Kalmar, Sweden.(Swepub:uu)krisnil (författare)
  • Nilsson, BoUppsala universitet,Vaskulärbiologi(Swepub:uu)bonils (författare)
  • Chung, Ung-ilUniv Tokyo, Sch Engn, Dept Bioengn, 7-3-1 Hongo,Bunkyo Ku, Tokyo 1138656, Japan. (författare)
  • Teramura, YujiUppsala universitet,Vaskulärbiologi,Natl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan.;Univ Tsukuba, Masters Doctoral Program Life Sci Innovat T LSI, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058577, Japan.(Swepub:uu)yujte186 (författare)
  • Univ Tokyo, Sch Engn, Dept Bioengn, 7-3-1 Hongo,Bunkyo Ku, Tokyo 1138656, Japan.Natl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan. (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Regenerative Therapy: Elsevier22, s. 90-982352-32042352-3204

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