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Phylogenetic analys...
Phylogenetic analysis of the non-structural (NS) gene of influenza A viruses isolated from mallards in Northern Europe in 2005
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Zohari, Siamak (författare)
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Gyarmati, P. (författare)
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Ejdersund, A. (författare)
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Berglöf, U. (författare)
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Thorén, P. (författare)
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Ehrenberg, M. (författare)
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Czifra, G. (författare)
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Belak, S. (författare)
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- Waldenström, Jonas (författare)
- Uppsala universitet,Högskolan i Kalmar,Naturvetenskapliga institutionen,Infektionssjukdomar
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- Olsen, Björn (författare)
- Uppsala universitet,Högskolan i Kalmar,Naturvetenskapliga institutionen,Infektionssjukdomar
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Berg, M. (författare)
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(creator_code:org_t)
- 2008
- 2008
- Engelska.
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Ingår i: Virology Journal. - 1743-422X. ; 5:Article ID: 147
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Although the important role of the non-structural 1 (NS) gene of influenza A in virulence of the virus is well established, our knowledge about the extent of variation in the NS gene pool of influenza A viruses in their natural reservoirs in Europe is incomplete. In this study we determined the subtypes and prevalence of influenza A viruses present in mallards in Northern Europe and further analysed the NS gene of these isolates in order to obtain a more detailed knowledge about the genetic variation of NS gene of influenza A virus in their natural hosts. Results: A total number of 45 influenza A viruses of different subtypes were studied. Eleven haemagglutinin-and nine neuraminidase subtypes in twelve combinations were found among the isolated viruses. Each NS gene reported here consisted of 890 nucleotides; there were no deletions or insertions. Phylogenetic analysis clearly shows that two distinct gene pools, corresponding to both NS allele A and B, were present at the same time in the same geographic location in the mallard populations in Northern Europe. A comparison of nucleotide sequences of isolated viruses revealed a substantial number of silent mutations, which results in high degree of homology in amino acid sequences. The degree of variation within the alleles is very low. In our study allele A viruses displays a maximum of 5% amino acid divergence while allele B viruses display only 2% amino acid divergence. All the viruses isolated from mallards in Northern Europe possessed the typical avian ESEV amino acid sequence at the C-terminal end of the NSI protein. Conclusion: Our finding indicates the existence of a large reservoir of different influenza A viruses in mallards population in Northern Europe. Although our phylogenetic analysis clearly shows that two distinct gene pools, corresponding to both NS allele A and B, were present in the mallards populations in Northern Europe, allele B viruses appear to be less common in natural host species than allele A, comprising only about 13% of the isolates sequenced in this study.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- Zoonotisk ekologi
- Zoonotic Ecology
- Mikrobiologi
- Microbiology
- Virology
- Virologi
- Infectious diseases
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Zohari, Siamak
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Gyarmati, P.
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Ejdersund, A.
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Berglöf, U.
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Thorén, P.
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Ehrenberg, M.
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visa fler...
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Czifra, G.
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Belak, S.
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Waldenström, Jon ...
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Olsen, Björn
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Berg, M.
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Mikrobiologi
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
- Artiklar i publikationen
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Virology Journal
- Av lärosätet
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Linnéuniversitetet
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Uppsala universitet