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Phylogenetic analys...
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Zohari, Siamak
(författare)
Phylogenetic analysis of the non-structural (NS) gene of influenza A viruses isolated from mallards in Northern Europe in 2005
- Artikel/kapitelEngelska2008
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:lnu-1914
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https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-1914URI
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https://doi.org/10.1186/1743-422X-5-147DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-88440URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Background: Although the important role of the non-structural 1 (NS) gene of influenza A in virulence of the virus is well established, our knowledge about the extent of variation in the NS gene pool of influenza A viruses in their natural reservoirs in Europe is incomplete. In this study we determined the subtypes and prevalence of influenza A viruses present in mallards in Northern Europe and further analysed the NS gene of these isolates in order to obtain a more detailed knowledge about the genetic variation of NS gene of influenza A virus in their natural hosts. Results: A total number of 45 influenza A viruses of different subtypes were studied. Eleven haemagglutinin-and nine neuraminidase subtypes in twelve combinations were found among the isolated viruses. Each NS gene reported here consisted of 890 nucleotides; there were no deletions or insertions. Phylogenetic analysis clearly shows that two distinct gene pools, corresponding to both NS allele A and B, were present at the same time in the same geographic location in the mallard populations in Northern Europe. A comparison of nucleotide sequences of isolated viruses revealed a substantial number of silent mutations, which results in high degree of homology in amino acid sequences. The degree of variation within the alleles is very low. In our study allele A viruses displays a maximum of 5% amino acid divergence while allele B viruses display only 2% amino acid divergence. All the viruses isolated from mallards in Northern Europe possessed the typical avian ESEV amino acid sequence at the C-terminal end of the NSI protein. Conclusion: Our finding indicates the existence of a large reservoir of different influenza A viruses in mallards population in Northern Europe. Although our phylogenetic analysis clearly shows that two distinct gene pools, corresponding to both NS allele A and B, were present in the mallards populations in Northern Europe, allele B viruses appear to be less common in natural host species than allele A, comprising only about 13% of the isolates sequenced in this study.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Gyarmati, P.
(författare)
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Ejdersund, A.
(författare)
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Berglöf, U.
(författare)
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Thorén, P.
(författare)
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Ehrenberg, M.
(författare)
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Czifra, G.
(författare)
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Belak, S.
(författare)
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Waldenström, JonasUppsala universitet,Högskolan i Kalmar,Naturvetenskapliga institutionen,Infektionssjukdomar(Swepub:lnu)ewajo
(författare)
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Olsen, BjörnUppsala universitet,Högskolan i Kalmar,Naturvetenskapliga institutionen,Infektionssjukdomar(Swepub:uu)bjool781
(författare)
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Berg, M.
(författare)
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Högskolan i KalmarNaturvetenskapliga institutionen
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Virology Journal5:Article ID: 1471743-422X
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Till lärosätets databas
- Av författaren/redakt...
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Zohari, Siamak
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Gyarmati, P.
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Ejdersund, A.
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Berglöf, U.
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Thorén, P.
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Ehrenberg, M.
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visa fler...
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Czifra, G.
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Belak, S.
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Waldenström, Jon ...
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Olsen, Björn
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Berg, M.
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visa färre...
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och Mikrobiologi
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
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Virology Journal
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