Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes

• Extracellular cellulolytic enzymes were produced under solid state cultivation by the thermophilic fungus Thermoascus aurantiacus and characterized. Elevated levels of endoglucanase and β-glucosidase activities were produced simultaneously by optimization of growth factors. Under optimal growth conditions, 1572 U endoglucanase and 101.6 U β-glucosidase per g of carbon source were obtained. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of glucose (MUG) and cellobiose (MUG2) were used to characterize the cellulolytic multienzyme components after separation by isoelectric focusing. The zymogram indicated one endoglucanase and one β-glucosidase with pI values 3.5 and 3.9, respectively. Both enzymes exhibited significant thermostability, with half-lives of 42 and 18 min, respectively, at 80°C

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)