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Optimization of β-g...
Optimization of β-glucosidase catalysed synthesis of trisaccharides from cellobiose and gentiobiose
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Christakopoulos, Paul (författare)
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- Kekos, Dimitris (författare)
- National Technical University of Athens
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- Macris, Basil J. (författare)
- National Technical University of Athens
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- Goodenough, Peter W. (författare)
- Institute of Food Research, Reading
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- Bhat, Mahalingeshwara K. (författare)
- Institute of Food Research, Reading
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(creator_code:org_t)
- 1994
- 1994
- Engelska.
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Ingår i: Biotechnology letters. - 0141-5492 .- 1573-6776. ; 16:6, s. 587-592
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Purified β-Glucosidase from Fusarium oxysporum catalysed the hydrolysis and transglycosylation reactions in the presence of cellobiose and gentiobiose. The product of the latter reaction was mainly a triose. The time of incubation, pH and substrate concentration for transglycosylation reaction were optimised. Under optimal conditions, the concentration of glucose and triose reached approximately 15–20 % of the initial substrate concentration. These results suggested that β-glucosidase from F.oxysporum is an ideal enzyme for the synthesis of triose in reasonable quantities.
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- art (ämneskategori)
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