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Optimized Enzymatic...
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Antonopoulou, Io,1989-Luleå tekniska universitet,Kemiteknik
(författare)
Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions
- Artikel/kapitelEngelska2018
Förlag, utgivningsår, omfång ...
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Elsevier,2018
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:ltu-71275
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https://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-71275URI
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https://doi.org/10.1016/j.csbj.2018.09.005DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Validerad;2018;Nivå 2;2018-10-18 (svasva)
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Three novel feruloyl esterases (Fae125, Fae7262 and Fae68) from Talaromyces wortmanniioverexpressed in the C1 platform were evaluated for the transesterification of vinyl ferulatewith two acceptors of different size and lipophilicity (prenol and L-arabinose) in detergentless microemulsions. The effect of reaction conditions such as the microemulsion composition, the substrate concentration, the enzyme load, the pH, the temperature and the agitation were investigated. The type A Fae125 belonging to the subfamily 5 (SF5) of phylogenetic classification showed highest yields for the synthesis of both products after optimization of reaction conditions: 81.8% for prenyl ferulate and 33.0% for L-arabinose ferulate. After optimization, an 8-fold increase in the yield and a 12-fold increase in selectivity were achieved for the synthesis of prenyl ferulate.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Iancu, LauraDupont Industrial Biosciences, Wageningen, the Netherlands
(författare)
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Jütten, PeterTaros Chemicals GmbH & Co KG, Dortmund, Germany
(författare)
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Piechot, AlexanderTaros Chemicals GmbH & Co KG, Dortmund, Germany
(författare)
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Rova, UlrikaLuleå tekniska universitet,Kemiteknik(Swepub:ltu)ulrok
(författare)
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Christakopoulos, PaulLuleå tekniska universitet,Kemiteknik(Swepub:ltu)pauchr
(författare)
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Luleå tekniska universitetKemiteknik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Computational and Structural Biotechnology Journal: Elsevier, s. 361-3692001-0370
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