Sökning: onr:"swepub:oai:DiVA.org:ltu-72926" >
Directed evolution ...
Directed evolution of the type C feruloyl esterase from Fusarium oxysporum FoFaeC and molecular docking analysis of its improved variants
-
- Cerullo, Gabriella (författare)
- University of Naples “Federico II”, Naples, Italy
-
- Varriale, Simona (författare)
- University of Naples “Federico II”, Naples, Italy
-
- Bozonnet, Sophie (författare)
- Université de Toulouse, Toulouse, France
-
visa fler...
-
- Antonopoulou, Io, 1989- (författare)
- Luleå tekniska universitet,Kemiteknik
-
- Christakopoulos, Paul (författare)
- Luleå tekniska universitet,Kemiteknik
-
- Rova, Ulrika (författare)
- Luleå tekniska universitet,Kemiteknik
-
- Gherbovet, Olga (författare)
- Université de Toulouse, Toulouse, France
-
- Fauré, Régis (författare)
- Université de Toulouse, Toulouse, France
-
- Piechot, Alexander (författare)
- Taros Chemicals GmbH & Co. KG, Dortmund, Germany
-
- Jütten, Peter (författare)
- Taros Chemicals GmbH & Co. KG, Dortmund, Germany
-
- Brás, Joana L.A. (författare)
- NzyTech LDA, Lisbon, Portugal
-
- Fontes, Carlos M.G.A. (författare)
- NzyTech LDA, Lisbon, Portugal
-
- Faraco, Vincenza (författare)
- University of Naples “Federico II”, Naples, Italy
-
visa färre...
-
(creator_code:org_t)
- Elsevier, 2019
- 2019
- Engelska.
-
Ingår i: New Biotechnology. - : Elsevier. - 1871-6784 .- 1876-4347. ; 51, s. 14-20
- Relaterad länk:
-
https://hal.insa-tou...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The need to develop competitive and eco-friendly processes in the cosmetic industry leads to the search for new enzymes with improved properties for industrial bioconversions in this sector. In the present study, a complete methodology to generate, express and screen diversity for the type C feruloyl esterase from Fusarium oxysporium FoFaeC was set up in a high-throughput fashion. A library of around 30,000 random mutants of FoFaeC was generated by error prone PCR of fofaec cDNA and expressed in Yarrowia lipolytica. Screening for enzymatic activity towards the substrates 5-bromo-4-chloroindol-3-yl and 4-nitrocatechol-1-yl ferulates allowed the selection of 96 enzyme variants endowed with improved enzymatic activity that were then characterized for thermo- and solvent- tolerance. The five best mutants in terms of higher activity, thermo- and solvent- tolerance were selected for analysis of substrate specificity. Variant L432I was shown to be able to hydrolyze all the tested substrates, except methyl sinapate, with higher activity than wild type FoFaeC towards methyl p-coumarate, methyl ferulate and methyl caffeate. Moreover, the E455D variant was found to maintain completely its hydrolytic activity after two hour incubation at 55 °C, whereas the L284Q/V405I variant showed both higher thermo- and solvent- tolerance than wild type FoFaeC. Small molecule docking simulations were applied to the five novel selected variants in order to examine the binding pattern of substrates used for enzyme characterization of wild type FoFaeC and the evolved variants.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Nyckelord
- Directed evolution
- Feruloyl esterase
- Fusarium oxysporum
- High-throughput screening
- Library
- Biokemisk processteknik
- Biochemical Process Engineering
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Cerullo, Gabriel ...
-
Varriale, Simona
-
Bozonnet, Sophie
-
Antonopoulou, Io ...
-
Christakopoulos, ...
-
Rova, Ulrika
-
visa fler...
-
Gherbovet, Olga
-
Fauré, Régis
-
Piechot, Alexand ...
-
Jütten, Peter
-
Brás, Joana L.A.
-
Fontes, Carlos M ...
-
Faraco, Vincenza
-
visa färre...
- Om ämnet
-
- TEKNIK OCH TEKNOLOGIER
-
TEKNIK OCH TEKNO ...
-
och Industriell biot ...
-
och Bioprocessteknik
- Artiklar i publikationen
-
New Biotechnolog ...
- Av lärosätet
-
Luleå tekniska universitet