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A Purified α-galact...
A Purified α-galactosidase from aspergillus niger with enhanced kinetic characteristics
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- Agnantiari, G. (författare)
- National Technical University of Athens
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Christakopoulos, Paul (författare)
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- Kekos, D. (författare)
- National Technical University of Athens
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- Macris, B.J. (författare)
- National Technical University of Athens
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(creator_code:org_t)
- Wiley, 1991
- 1991
- Engelska.
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Ingår i: Acta Biotechnologica. - : Wiley. - 0138-4988 .- 1521-3846. ; 11:5, s. 479-484
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Extracellular α-galactosidase from Aspergillus niger was purified 128-fold over the crude extract by gel filtration, ion exchange chromatography and chromatofocusing. Certain substrates and end products affected enzyme activity. Among the former p-nitrophenyl-α-galactopyranoside (PNPG) inhibited the enzyme at 1.4 mM while melibiose did not inhibit α-galactosidase at concentrations up to 50 mM. Enzymic end products such as glucose did not inhibit the enzyme at concentrations up to 100 mM while galactose exhibited a competitive inhibition with a Ki = 1.29 mM. The kinetic characteristics of the enzyme compared favourably to other microbial α-galactosidases and make it suitable for food process applications.
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