Sökning: onr:"swepub:oai:DiVA.org:mau-15860" >
Role for the A Doma...
Role for the A Domain of Unprocessed Accumulation-Associated Protein (Aap) in the Attachment Phase of the Staphylococcus epidermidis Biofilm Phenotype
-
Conlon, Brian (författare)
-
Geoghegan, Joan (författare)
-
Waters, Elaine (författare)
-
visa fler...
-
McCarthy, Hannah (författare)
-
Rowe, S (författare)
-
- Davies, Julia R (författare)
- Malmö högskola,Odontologiska fakulteten (OD)
-
Schaeffer, Carolyn (författare)
-
Foster, Timothy (författare)
-
Fey, Paula (författare)
-
O'Gara, James P (författare)
-
visa färre...
-
(creator_code:org_t)
- American Society for Microbiology, 2014
- 2014
- Engelska.
-
Ingår i: Journal of Bacteriology. - : American Society for Microbiology. - 0021-9193 .- 1098-5530. ; 196:24, s. 4268-4275
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The polysaccharide intercellular adhesin or the cell wall-anchored accumulation-associated protein (Aap) mediates cellular accumulation during Staphylococcus epidermidis biofilm maturation. Mutation of sortase, which anchors up to 11 proteins (including Aap) to the cell wall, blocked biofilm development by the cerebrospinal fluid isolate CSF41498. Aap was implicated in this phenotype when Western blots and two-dimensional (2D) electrophoresis revealed increased levels of the protein in culture supernatants. Unexpectedly, reduced levels of primary attachment were associated with impaired biofilm formation by CSF41498 srtA and aap mutants. In contrast to previous studies, which implicated Aap proteolytic cleavage and, specifically, the Aap B domains in biofilm accumulation, the CSF41498 Aap protein was unprocessed. Furthermore, aap appeared to play a less important role in the biofilm phenotype of S. epidermidis 1457, in which the Aap protein is processed. Anti-Aap A-domain IgG inhibited primary attachment and biofilm formation in strain CSF41498 but not in strain 1457. The nucleotide sequences of the aap gene A-domain region and cleavage site in strains CSF41498 and 1457 were identical, implicating altered protease activity in the differential Aap processing results in the two strains. These data reveal a new role for the A domain of unprocessed Aap in the attachment phase of biofilm formation and suggest that extracellular protease activity can influence whether Aap contributes to the attachment or accumulation phases of the S. epidermidis biofilm phenotype
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- biofilm
- SrtA
- LPXTG proteins
- Aap
- primary attachment
- Staphylococcus epidermidis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Conlon, Brian
-
Geoghegan, Joan
-
Waters, Elaine
-
McCarthy, Hannah
-
Rowe, S
-
Davies, Julia R
-
visa fler...
-
Schaeffer, Carol ...
-
Foster, Timothy
-
Fey, Paula
-
O'Gara, James P
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Odontologi
- Artiklar i publikationen
-
Journal of Bacte ...
- Av lärosätet
-
Malmö universitet