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Sökning: onr:"swepub:oai:DiVA.org:mau-53384" > Streptococcus gordo...

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FältnamnIndikatorerMetadata
00004994naa a2200433 4500
001oai:DiVA.org:mau-53384
003SwePub
008220622s2022 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-533842 URI
0247 a https://doi.org/10.1128/jb.00118-222 DOI
040 a (SwePub)mau
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Lima, Bruno P.u Univ Minnesota, Sch Dent, Dept Diagnost & Biol Sci, Minneapolis, MN 55455 USA.4 aut
2451 0a Streptococcus gordonii Poised for Glycan Feeding through a MUC5B-Discriminating, Lipoteichoic Acid-Mediated Outside-In Signaling Circuit
264 1b ASM International,c 2022
338 a print2 rdacarrier
520 a Many oral bacteria employ cell wall-anchored adhesins to bind to the salivary films coating the teeth and mucosal surfaces. Surface binding prevents clearance and facilitates catabolism of salivary film glycoproteins. We asked whether Streptococcus gordonii adhesin expression changes in response to surface salivary cues using a eukaryote-like, outside-in recognition and signaling circuit. To determine whether the cues were discriminated, S. gordonii was tested during cell adhesion and biofilm formation on a MUC5B-rich or lower-molecular-mass salivary fraction or an uncoated abiotic surface. Cells were recovered and analyzed for differences in gene expression and proteins in cell wall fractions. In salivary-free conditions, planktonic S. gordonii presented three prominent cell wall LPXTG-motif proteins, SGO_1487, SGO_0890, and MbpA (mucin-binding protein A; SGO_0707). During biofilm formation on MUC5B-coated surfaces, MbpA, a MUC5B-binding protein, and key genes in the tagatose and quorum-sensing pathways were strongly promoted. The response to MUC5B required the two-component system (TCS), streptococcal regulator of adhesins sensor and regulator (SraSR, SGO_1180/81), lipoteichoic acid (LTA), and the homologous paired adhesins, SspA and SspB (SspAB). LTA appears to link the outside signal (MUC5B) to intramembrane SraSR. Tagatose pathway gene expression may poise cells to metabolize MUC5B glycans and, with a quorum-sensing gene (luxS), may direct formation of a consortium to facilitate glycan cross-feeding by S. gordonii. We now show that a Gram-positive bacterium discriminates specific surface environmental cues using an outside-in signaling mechanism to apparently optimize colonization of saliva-coated surfaces. IMPORTANCE All organisms throughout the tree of life sense and respond to their surface environments. To discriminate among mucosal surface environmental cues, we report that Streptococcus gordonii recognizes a high-molecular-weight mucin glycoprotein, MUC5B, using the paired adhesins SspAB and lipoteichoic acid; the latter bridges the outside signal to an intramembrane two-component system to transcriptionally regulate a MUC5B-specific adhesin and genes that may facilitate glycan catabolism. All organisms throughout the tree of life sense and respond to their surface environments. To discriminate among mucosal surface environmental cues, we report that Streptococcus gordonii recognizes a high-molecular-weight mucin glycoprotein, MUC5B, using the paired adhesins SspAB and lipoteichoic acid; the latter bridges the outside signal to an intramembrane two-component system to transcriptionally regulate a MUC5B-specific adhesin and genes that may facilitate glycan catabolism.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Odontologi0 (SwePub)302162 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Dentistry0 (SwePub)302162 hsv//eng
653 a Streptococcus gordonii
653 a signaling circuit
653 a glycan feeding
653 a MUC5B
653 a adhesins
653 a lipoteichoic acid
653 a two-component system
7001 a Davies, Julia R,d 1962-u Malmö universitet,Odontologiska fakulteten (OD)4 aut0 (Swepub:mau)odjuda
7001 a Wickström, Claes,d 1970-u Malmö universitet,Odontologiska fakulteten (OD)4 aut0 (Swepub:mau)odclwi
7001 a Johnstone, Karen F.u Univ Minnesota, Sch Dent, Dept Diagnost & Biol Sci, Minneapolis, MN 55455 USA.4 aut
7001 a Hall, Jeffrey W.u Univ Minnesota, Sch Dent, Dept Diagnost & Biol Sci, Minneapolis, MN 55455 USA.4 aut
7001 a Svensäter, Gunnel,d 1954-u Malmö universitet,Odontologiska fakulteten (OD)4 aut0 (Swepub:mau)odgusv
7001 a Herzberg, Mark C.u Univ Minnesota, Sch Dent, Dept Diagnost & Biol Sci, Minneapolis, MN 55455 USA.4 aut
7102 a Univ Minnesota, Sch Dent, Dept Diagnost & Biol Sci, Minneapolis, MN 55455 USA.b Odontologiska fakulteten (OD)4 org
7730 t Journal of Bacteriologyd : ASM Internationalg 204:6q 204:6x 0021-9193x 1098-5530
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-53384
8564 8u https://doi.org/10.1128/jb.00118-22

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