Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applications

2000-11-03
Univ Gothenburg, Dept Chem, SE-41296 Gothenburg, Sweden. Univ Gothenburg, Dept Chem Phys, SE-41345 Gothenburg, Sweden.AMER CHEMICAL SOC,2000
printrdacarrier

A combined electroporation and pressure-driven microinjection method for efficient loading of biopolymers and colloidal particles into single-cell-sized unilamellar liposomes was developed. Single liposomes were positioned between a similar to2-mum tip diameter solute-filled glass micropipet, equipped with a Pt electrode, and a 5-mum-diameter carbon fiber electrode. A transient, 1-10 ms, rectangular waveform de voltage pulse (10-40 V/cm) was applied between the electrodes, thus focusing the electric field over the liposome. Dielectric membrane breakdown induced by the applied voltage pulse caused the micropipet tip to enter the liposome and a small volume (typically 50-500 x 10(-15) L) of fluorescein, YOYO-intercalated T7-phage DNA, 100-nm-diameter unilamellar liposomes, or fluorescent latex spheres could be injected into the intraliposomal compartment. We also demonstrate initiation of a chemical intercalation reaction between T2-phage DNA and YOYO-1 by dual injection into a single giant unilamellar liposome. The method was also successfully applied for loading of single cultured cells.

Nolkrantz, KDepartment of Chemistry, Göteborg University (författare)
Davidson, M JDepartment of Chemistry, Göteborg University (författare)
Strömberg, AnetteDepartment of Chemistry, Göteborg University(Swepub:mdh)asg03 (författare)
Ryttsen, FDepartment of Chemistry, Göteborg University (författare)
Akerman, BDepartment of Chemistry, Göteborg University (författare)
Orwar, ODepartment of Chemistry, Göteborg University (författare)
Department of Chemistry, Göteborg University (creator_code:org_t)

Ingår i:Analytical ChemistryUniv Gothenburg, Dept Chem, SE-41296 Gothenburg, Sweden. Univ Gothenburg, Dept Chem Phys, SE-41345 Gothenburg, Sweden. : AMER CHEMICAL SOC72:23, s. 5857-58620003-27001520-6882

Av författaren/redakt...: Karlsson, M; Nolkrantz, K; Davidson, M J; Strömberg, Anett ...; Ryttsen, F; Akerman, B; visa fler...; Orwar, O; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.