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- Kälvegren, HannaÖstergötlands Läns Landsting,Linköpings universitet,Örebro universitet,Hälsoakademin,Kardiologi,Hälsouniversitetet,Kardiologiska kliniken US,University of Örebro (författare)
Release of matrix metalloproteinases-1 and-2, but not-9, from activated platelets measured by enzyme-linked immunosorbent assay
- Artikel/kapitelEngelska2011
Förlag, utgivningsår, omfång ...
- 2011-07-14
- Informa Healthcare,2011
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:oru-20814
- https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-20814URI
- https://doi.org/10.3109/09537104.2011.583300DOI
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-72150URI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
Klassifikation
Anmärkningar
- Matrix metalloproteinases (MMPs), in particular MMP-9, have been introduced as novel biomarkers in coronary artery disease. Activated platelets are considered to be a major source of the highly elevated levels of MMPs that are detected in serum compared to plasma. The aim of this study was to clarify if activated platelets release MMPs-1, -2 and -9 as measured by enzyme-linked immunosorbent assays (ELISA). Isolated platelets (separated by several procedures) or platelet-rich plasma (PRP) were stimulated by collagen, thrombin or the TLR2 agonist Pam(3)CSK(4). The concentrations of MMPs-1,-2 and -9 in supernatants were determined by ELISA. In addition, a MMP-9 enzyme activity assay was used as well as immunofluorescent staining of MMPs-1,-2 and 9 in platelets. Isolated platelets stimulated by collagen, thrombin or Pam(3)CSK(4) released significant amounts of MMP-1 to the supernatant measured as either pro- or total-MMP-1. However, there was no detectable release of MMP-2 or -9 from isolated platelets. Collagen-stimulated platelets in PRP released MMP-2, but not -9. Before stimulation; platelets were positive for MMPs-1 and -2, but not -9, as assessed by immunofluorescence. Acting as positive controls, neutrophils were found to release significant amounts of MMP-9. Our findings indicate that activated platelets may be a major source of MMP-1 and to a minor extent MMP-2, in peripheral blood. However, in contrast to what has been argued in previous literature, platelets appear to be only negligible contributors to circulating MMP-9.
Ämnesord och genrebeteckningar
- MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Biomedicinsk laboratorievetenskap/teknologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Medical Biotechnology Biomedical Laboratory Science/Technology hsv//eng
- Matrix metalloproteinase
- platelets
- Biomedicin
- Biomedicine
- MEDICINE
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Jonsson, SimonÖrebro universitet,Hälsoakademin (författare)
- Jonasson, LenaÖstergötlands Läns Landsting,Linköpings universitet,Örebro universitet,Hälsoakademin,Hälsouniversitetet,Avdelningen för kardiovaskulär medicin,Kardiologiska kliniken US(Swepub:liu)lenjo59 (författare)
- Örebro universitetHälsoakademin (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Platelets: Informa Healthcare22:8, s. 572-5780953-71041369-1635
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- Kälvegren, Hanna
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