Lipopolysaccharide-induced cytokine and chemokine expression in human carotid lesions

Jatta, KenÖrebro universitet,Institutionen för klinisk medicin,University of Örebro, Sweden (författare)

The release of cytokines and chemokines from activated immune-competent cells plays a crucial role in determining the pathology of the atherogenic progress. We investigated the effect of bacterial lipopolysaccharide (LPS) on cytokine/chemokine expression in carotid lesions and normal renal arteries. The lesions or renal arteries were incubated for 6 h at 37 degrees C in serum-free media treated with or without LPS. After LPS treatment, increased protein levels of IL-1beta, IL-6, IL-8, IL-10, TNF-alpha and MCP-1 were observed in the culture medium from the lesions measured with cytometric bead array. We were able to detect the induction of IL-1beta, IL-6, IL-8, IL-10, TNF-alpha and MCP-1 mRNA in the lesions after stimulation with LPS using real-time PCR. In renal arteries, LPS also induces mRNA expression of all chemokines and cytokines investigated with the exception of IL-6. However, LPS induces significantly higher levels of TNF-alpha, IL-1beta and IL-10 mRNA in lesions compared to renal arteries. The results suggest that infectious agents are capable of enhancing the production of cytokines/chemokines in an already ongoing inflammatory process such as in atherosclerosis, and that low levels of circulating LPS may affect the levels of pro-inflammatory cytokines much more in atherosclerotic vessels than in normal vessels and may contribute to the development of the atherosclerotic lesion.

Wågsäter, DickÖrebro universitet,Institutionen för klinisk medicin,University of Örebro, Sweden(Swepub:liu)dicwa60 (författare)
Norgren, LarsÖrebro University Hospital, Sweden (författare)
Stenberg, BjörnÖrebro University Hospital, Sweden (författare)
Sirsjö, AllanÖrebro universitet,Institutionen för klinisk medicin,University of Örebro, Sweden(Swepub:oru)aso (författare)
Örebro universitetInstitutionen för klinisk medicin (creator_code:org_t)

Ingår i:Journal of Vascular Research: S. Karger AG42:3, s. 266-2711018-11721423-0135

Av författaren/redakt...: Jatta, Ken; Wågsäter, Dick; Norgren, Lars; Stenberg, Björn; Sirsjö, Allan

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