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Determination of ch...
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Ekblad, Alf,1957-University of Umeå, Umeå, Sweden
(författare)
Determination of chitin in fungi and mycorrhizal roots by an improved HPLC analysis of glucosamine
- Artikel/kapitelEngelska1996
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LIBRIS-ID:oai:DiVA.org:oru-37412
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-37412URI
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https://doi.org/10.1007/BF00011160DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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A method to measure chitin content in fungi and ectomycorrhizal roots with high-performance liquid chromatography (HPLC) was developed. Measurements of fluorescence of 9-fluorenylmethylchloroformate (FMOC-CI) derivatives of glucosamine were made on acid hydrolysates of pure chitin, chitin-root mixtures and fungal-root mixtures. The method was applied on 5 isolates of ectomycorrhizal fungi, and ectomycorrhizal and non-mycorrhizal Pinus sylvestris roots. Interference from amino acids was removed by pre-treatment of samples with 0.2 N NaOH. This pre-treatment did not reduce the recovery of chitin, nor did plant material affect the recovery of chitin. The HPLC method was compared with a colorimetric chitin-method by measurements on root-fungal mixtures, with known fungal content. The HPLC method gave estimates of fungal biomass which were equal to the expected while the colorimetric method showed values significantly (p<0.001) lower than the expected. The present chitin method offers a sensitive and specific tool for the quantification of chitin in fungi and in ectomycorrhizal roots.
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Näsholm, TorgnySwedish University of Agricultural Sciences, Umeå, Sweden
(författare)
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University of Umeå, Umeå, SwedenSwedish University of Agricultural Sciences, Umeå, Sweden
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Plant and Soil178:1, s. 29-350032-079X1573-5036
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