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A combined culture ...
A combined culture and PCR method for detection of pathogenic Yersinia enterocolitica in food
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- Thisted Lambertz, Susanne (författare)
- National Food Administration, Uppsala, Sweden
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- Lindqvist, Roland (författare)
- National Food Administration, Uppsala, Sweden
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- Ballagi-Pordany, A (författare)
- Microbiology Department, Uppsala University, Uppsala, Sweden
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- Danielsson-Tham, Marie-Louise, 1947- (författare)
- Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden
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(creator_code:org_t)
- 2000
- 2000
- Engelska.
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Ingår i: International Journal of Food Microbiology. - 0168-1605 .- 1879-3460. ; 57:1-2, s. 63-73
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A combined method based on traditional culturing, buoyant density centrifugation, (BDC), and polymerase chain reaction (PCR) techniques for detection and identification of pathogenic Y. enterocolitica in food was developed and evaluated. An internal control, which was added in each PCR-tube and co-amplified by the same primer pair as the pathogen, monitored false-negative PCR results. The sample preparation step, BDC, was used to remove PCR inhibiting food substances and to concentrate the Y. enterocolitica cells. Single PCR with a chromosomal gene (ail) as target was chosen for screening the samples. The method was tested on naturally and artificially contaminated food samples. In three different food samples, processed meat (brawn), unprocessed beef and minced pork, inoculated with 10 cfu pathogenic Y. enterocolitica per gram, Y. enterocolitica was detected and cultural bacteria indicated within 18 h of enrichment.
Ämnesord
- SAMHÄLLSVETENSKAP -- Annan samhällsvetenskap -- Övrig annan samhällsvetenskap (hsv//swe)
- SOCIAL SCIENCES -- Other Social Sciences -- Other Social Sciences not elsewhere specified (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Buoyant density centrifugation; Food; Pathogenic Y. enterocolitica; PCR
- Måltidskunskap
- Culinary Arts and Meal Science
- Microbiology
- Mikrobiologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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