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Detection of Yersin...
Detection of Yersinia enterocolitica in food by PCR amplification
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- Nilsson, Anders (författare)
- National Food Administration, Swedish University of Agricultural Sciences, Uppsala
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- Lambertz, Susanne (författare)
- National Food Administration, Swedish University of Agricultural Sciences, Uppsala
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- Stålhandske, Per (författare)
- Department of Food Hygiene, Swedish University of Agricultural Sciences, Uppsala
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- Norberg, Per (författare)
- National Food Administration, Swedish University of Agricultural Sciences, Uppsala
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- Danielsson-Tham, Marie-Louise, 1947- (författare)
- Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden
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(creator_code:org_t)
- Oxon, United Kingdom : Blackwell Publishing, 1998
- 1998
- Engelska.
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Ingår i: Letters in Applied Microbiology. - Oxon, United Kingdom : Blackwell Publishing. - 0266-8254 .- 1472-765X. ; 26, s. 140-144
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A polymerase chain reaction (PCR) assay was developed for detection ofpathogenic, virulent strains of Yersinia enterocolitica. By using both virulence loci virFand ail as markers for pathogenicity, detection of species with a virulence factor present waspossible. DNA preparation in the presence of hexadecyl trimethy ammonium bromide(CTAB) was followed by two 44 cycle amplification reactions, one for each of themarkers. As few as 102Y. enterocolitica cells were detected in ground pork in thepresence of 105–106bacteria of other species. The described PCR assay providesa sensitive robust assay for the detection of virulent Y. enterocolitica in food.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- Måltidskunskap
- Culinary Arts and Meal Science
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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