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WGS analysis and mo...
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Jacobsson, Susanne,1974-Örebro universitet,Institutionen för medicinska vetenskaper
(författare)
WGS analysis and molecular resistance mechanisms of azithromycin-resistant (MIC >2 mg/L) Neisseria gonorrhoeae isolates in Europe from 2009 to 2014
- Artikel/kapitelEngelska2016
Förlag, utgivningsår, omfång ...
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2016-07-17
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Oxford, United Kingdom :Oxford University Press,2016
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:oru-51527
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-51527URI
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https://doi.org/10.1093/jac/dkw279DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Funding Agencies:Örebro County Council Research CommitteeFoundation for Medical Research at Örebro University Hospital, Sweden
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Objectives: To elucidate the genome-based epidemiology and phylogenomics of azithromycin-resistant (MIC >2 mg/L) Neisseria gonorrhoeae strains collected in 2009-14 in Europe and clarify the azithromycin resistance mechanisms.Methods: Seventy-five azithromycin-resistant (MIC 4 to >256 mg/L) N. gonorrhoeae isolates collected in 17 European countries during 2009-14 were examined using antimicrobial susceptibility testing and WGS.Results: Thirty-six N. gonorrhoeae multi-antigen sequence typing STs and five phylogenomic clades, including 4-22 isolates from several countries per clade, were identified. The azithromycin target mutation A2059G (Escherichia coli numbering) was found in all four alleles of the 23S rRNA gene in all isolates with high-level azithromycin resistance (n = 4; MIC ≥256 mg/L). The C2611T mutation was identified in two to four alleles of the 23S rRNA gene in the remaining 71 isolates. Mutations in mtrR and its promoter were identified in 43 isolates, comprising isolates within the whole azithromycin MIC range. No mutations associated with azithromycin resistance were found in the rplD gene or the rplV gene and none of the macrolide resistance-associated genes [mef(A/E), ere(A), ere(B), erm(A), erm(B), erm(C) and erm(F)] were identified in any isolate.Conclusions: Clonal spread of relatively few N. gonorrhoeae strains accounts for the majority of the azithromycin resistance (MIC >2 mg/L) in Europe. The four isolates with high-level resistance to azithromycin (MIC ≥256 mg/L) were widely separated in the phylogenomic tree and did not belong to any of the main clades. The main azithromycin resistance mechanisms were the A2059G mutation (high-level resistance) and the C2611T mutation (low- and moderate-level resistance) in the 23S rRNA gene.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Golparian, Daniel,1984-Örebro universitet,Institutionen för medicinska vetenskaper(Swepub:oru)degn
(författare)
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Cole, MichellePublic Health England, London, UK
(författare)
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Spiteri, GianfrancoEuropean Centre for Disease Prevention and Control, Stockholm, Sweden
(författare)
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Martin, IrenePublic Health Agency of Canada, Winnipeg, Canada
(författare)
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Bergheim, TheaOslo University Hospital Ullevål, Oslo, Norway
(författare)
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Borrego, Maria JoséNational Institute of Health, Lisbon, Portugal
(författare)
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Crowley, BrendanSt James’s Hospital, Dublin, Ireland
(författare)
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Crucitti, TaniaInstitute of Tropical Medicine, Antwerp, Belgium
(författare)
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Van Dam, Alje P.Public Health Service Amsterdam, Amsterdam, The Netherlands
(författare)
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Hoffmann, SteenStatens Serum Institut, Copenhagen, Denmark
(författare)
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Jeverica, SamoInstitute of Microbiology and Immunology, University of Ljubljana, Ljubljana, Slovenia
(författare)
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Kohl, PeterVivantes Klinikum Neukölln, Berlin, Germany
(författare)
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Mlynarczyk-Bonikowska, BeataMedical University of Warsaw, Warsaw, Poland
(författare)
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Pakarna, GatisInfectology Centre of Latvia, Riga, Latvia
(författare)
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Stary, AngelikaOutpatients’ Centre for Infectious Venereodermatological Diseases, Vienna, Austria
(författare)
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Stefanelli, PaolaIstituto Superiore di Sanitá, Rome, Italy
(författare)
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Pavlik, PeterHPL Laboratory Ltd, Bratislava, Slovakia
(författare)
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Tzelepi, EvaHellenic Pasteur Institute, Athens, Greece
(författare)
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Abad, RaquelInstitute of Health Carlos III, Madrid, Spain
(författare)
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Harris, Simon R.Pathogen Genomics, Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Hinxton, UK
(författare)
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Unemo, Magnus,1970-Örebro universitet,Institutionen för hälsovetenskaper(Swepub:oru)muo
(författare)
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Örebro universitetInstitutionen för medicinska vetenskaper
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Antimicrobial ChemotherapyOxford, United Kingdom : Oxford University Press71:11, s. 3109-31160305-74531460-2091
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Jacobsson, Susan ...
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Golparian, Danie ...
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Cole, Michelle
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Spiteri, Gianfra ...
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Martin, Irene
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Bergheim, Thea
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visa fler...
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Borrego, Maria J ...
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Crowley, Brendan
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Crucitti, Tania
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Van Dam, Alje P.
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Hoffmann, Steen
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Jeverica, Samo
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Kohl, Peter
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Mlynarczyk-Bonik ...
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Pakarna, Gatis
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Stary, Angelika
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Stefanelli, Paol ...
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Pavlik, Peter
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Tzelepi, Eva
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Abad, Raquel
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Harris, Simon R.
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Unemo, Magnus, 1 ...
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