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Sökning: onr:"swepub:oai:DiVA.org:oru-625" > Vitamin B6 as a pot...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003546nam a2200421 4500
001oai:DiVA.org:oru-625
003SwePub
008060918s2006 | |||||||||||000 ||eng|
020 a 9176684970q print
024a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-6252 URI
040 a (SwePub)oru
041 a engb eng
042 9 SwePub
072 7a vet2 swepub-contenttype
072 7a dok2 swepub-publicationtype
100a Ristilä, Mikaelu Örebro universitet,Institutionen för naturvetenskap4 aut0 (Swepub:oru)mlra
2451 0a Vitamin B6 as a potential antioxidant :b a study emanating from UV-B-stressed plants
264 1a Örebro :b Örebro universitetsbibliotek,c 2006
300 a 73 s.
338 a print2 rdacarrier
490a Örebro Studies in Life Science,x 1653-3100 ;v 3
520 a Increased influx of solar UV-B radiation (280-320 nm), due to a decreasing stratospheric ozone layer, impacts severely on plants. Some of the UV-B effects on plants are related to stress induced by produced reactive oxygen species (ROS). Pyridoxine (vitamin B6) has been reported to be a potential quencher of ROS.Previous studies have shown that the PDX1.3 gene (encoding a key enzyme in vitamin B6 biosynthesis) is up-regulated by UV-B. We showed that this up-regulation also occurs at the protein level in UV-B exposed Col-0 wild-type plants. Studies performed using pdx1 knock-out mutants of Arabidopsis thaliana showed elevated transcripts levels for LHCB1*3 and PR-5 compared with the Col-0 wild-type. The pdx1 knock-out mutants showed an increased sensitivity towards H2O2-stress, but no increased sensitivity towards low dose UV-B-stress.To study the postulated role of pyridoxine as quencher of ROS in more detail, both theoretical and experimental studies were performed. Theoretical and experimental UV absorption spectra obtained at different protonation/deprotonation states of pyridoxine showed a very close resemblance at low and high pH. However, for the computational studies at physiological pH addition of counterions were required to accurately describe the experimental spectra.When theoretically studying the reactivity of pyridoxine towards three different ROS, .OH, .OOH and .O2-, the hydroxyl radical (.OH) was shown to be the most reactive species, while the superoxide radical (.O2-) showed no reactivity towards pyridoxine.In order to study the role of G-proteins in UV-B signal transduction pathways, UV-B irradiated G-protein mutants of Arabidopsis thaliana were studied. The regulation of the PDX1.3 gene was not affected in the G-protein mutants, regardless of the developmental stage of the plant. However, the expression of CHS was affected in the Ga subunit mutants.
650 7a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe
650 7a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng
653 a Arabidopsis
653 a UV-B irradiation
653 a pyridoxine
653 a vitamin B6
653 a ROS
653 a Chemistry
653 a Kemi
653 a Chemistry
653 a Kemi
700a Strid, Åke,c Professoru Örebro universitet,Institutionen för naturvetenskap4 ths
700a Eriksson, Leif,c Professoru Örebro universitet,Institutionen för naturvetenskap4 ths
700a Hideg, Éva,c Professoru Institute of plant biology, Biological research center, Szeged, Ungern4 opn
710a Örebro universitetb Institutionen för naturvetenskap4 org
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-625

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