SwePub
Sök i LIBRIS databas

  Utökad sökning

onr:"swepub:oai:DiVA.org:oru-62836"
 

Sökning: onr:"swepub:oai:DiVA.org:oru-62836" > Prevalence of Flp P...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005988naa a2200565 4500
001oai:DiVA.org:oru-62836
003SwePub
008171127s2017 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-628362 URI
0247 a https://doi.org/10.3389/fmicb.2017.022412 DOI
040 a (SwePub)oru
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Davidsson, Sabina,d 1972-u Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Urology, Örebro University Hospital, Örebro, Sweden4 aut0 (Swepub:oru)sdn
2451 0a Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue
264 c 2017-11-16
264 1b Frontiers Media S.A.c 2017
338 a print2 rdacarrier
500 a Funding Agencies:Foundation for Medical Research at Örebro University Hospital  OLL-547931 Danish Medical Research council  DFF-1331-00241 
520 a Inflammation is one of the hallmarks of prostate cancer. The origin of inflammation is unknown, but microbial infections are suspected to play a role. In previous studies, the Gram-positive, low virulent bacterium Cutibacterium (formerly Propionibacterium) acnes was frequently isolated from prostatic tissue. It is unclear if the presence of the bacterium represents a true infection or a contamination. Here we investigated Cutibacterium acnes type II, also called subspecies defendens, which is the most prevalent type among prostatic C. acnes isolates. Genome sequencing of type II isolates identified large plasmids in several genomes. The plasmids are highly similar to previously identified linear plasmids of type I C. acnes strains associated with acne vulgaris. A PCR-based analysis revealed that 28.4% (21 out of 74) of all type II strains isolated from cancerous prostates carry a plasmid. The plasmid shows signatures for conjugative transfer. In addition, it contains a gene locus for tight adherence (tad) that is predicted to encode adhesive Flp (fimbrial low-molecular weight protein) pili. In subsequent experiments a tad locus-encoded putative pilin subunit was identified in the surface-exposed protein fraction of plasmid-positive C. acnes type II strains by mass spectrometry, indicating that the tad locus is functional. Additional plasmid-encoded proteins were detected in the secreted protein fraction, including two signal peptide-harboring proteins; the corresponding genes are specific for type II C. acnes, thus lacking from plasmid-positive type I C. acnes strains. Further support for the presence of Flp pili in C. acnes type II was provided by electron microscopy, revealing cell appendages in tad locus-positive strains. Our study provides new insight in the most prevalent prostatic subspecies of C. acnes, subsp. defendens, and indicates the existence of Flp pili in plasmid-positive strains. Such pili may support colonization and persistent infection of human prostates by C. acnes.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng
653 a Cutibacterium acnes
653 a Propionibacterium acnes
653 a plasmid
653 a fimbrial low-molecular weight protein
653 a pili
653 a tight adherence
653 a prostate cancer
7001 a Carlsson, Jessica,d 1984-u Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Urology, Örebro University Hospital, Örebro, Sweden4 aut0 (Swepub:oru)jccn
7001 a Mölling, Paulau Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden4 aut
7001 a Gashi, Natyrau Department of Urology, Örebro University Hospital, Örebro, Sweden4 aut
7001 a Andrén, Ove,d 1963-u Örebro universitet,Institutionen för medicinska vetenskaper,Department of Urology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden4 aut0 (Swepub:oru)oan
7001 a Andersson, Swen-Olofu Department of Urology, Örebro University Hospital, Örebro, Sweden4 aut
7001 a Brzuszkiewicz, Elzbietau Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, University of Göttingen, Göttingen, Germany4 aut
7001 a Poehlein, Anjau Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, University of Göttingen, Göttingen, Germany4 aut
7001 a Al-Zeer, Munir A.u Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany4 aut
7001 a Brinkmann, Volkeru Microscopy Core Facility, Max Planck Institute for Infection Biology, Berlin, Germany4 aut
7001 a Scavenius, Carstenu Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark4 aut
7001 a Nazipi, Sevenu Department of Biomedicine, Aarhus University, Aarhus, Denmark4 aut
7001 a Söderquist, Bo,d 1955-u Örebro universitet,Institutionen för medicinska vetenskaper,Department of Laboratory Medicine, Clinical Microbiology, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden4 aut0 (Swepub:oru)bost
7001 a Brüggemann, Holgeru Department of Biomedicine, Aarhus University, Aarhus, Denmark4 aut
7102 a Örebro universitetb Institutionen för medicinska vetenskaper4 org
7730 t Frontiers in Microbiologyd : Frontiers Media S.A.g 8q 8x 1664-302X
8564 u https://doi.org/10.3389/fmicb.2017.02241y Fulltext
8564 u https://www.frontiersin.org/articles/10.3389/fmicb.2017.02241/pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-62836
8564 8u https://doi.org/10.3389/fmicb.2017.02241

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy