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Spatial distributio...
Spatial distribution of glycerophospholipids in the ocular lens
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- Pól, J. (författare)
- Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Division of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland
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- Vidová, V. (författare)
- Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic
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- Hyötyläinen, Tuulia, 1971- (författare)
- VTT Technical Research Centre of Finland, Espoo, Finland
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- Volný, M. (författare)
- Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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- Novák, P. (författare)
- Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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- Strohalm, M. (författare)
- Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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- Kostiainen, R. (författare)
- Division of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland
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- Havlíček, V. (författare)
- Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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- Wiedmer, S. K. (författare)
- Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland
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- Holopainen, J. M. (författare)
- Helsinki Eye Lab, Department of Ophthalmology, University of Helsinki, Helsinki, Finland
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(creator_code:org_t)
- 2011-04-29
- 2011
- Engelska.
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Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 6:4
- Relaterad länk:
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https://doi.org/10.1...
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https://journals.plo...
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Abstract
Ämnesord
Stäng
- Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
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Pól, J.
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Vidová, V.
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Hyötyläinen, Tuu ...
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Volný, M.
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Novák, P.
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Strohalm, M.
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Kostiainen, R.
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Havlíček, V.
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Wiedmer, S. K.
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Holopainen, J. M ...
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Örebro universitet