Sökning: onr:"swepub:oai:DiVA.org:oru-65700" >
Novel, dynamic on-l...
Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles
-
- Helle, Anne (författare)
- Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland
-
- Hirsjärvi, Samuli (författare)
- Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland
-
- Peltonen, Leena (författare)
- Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland
-
visa fler...
-
- Hirvonen, Jouni (författare)
- Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland
-
- Wiedmer, Susanne K. (författare)
- Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland
-
- Hyötyläinen, Tuulia, 1971- (författare)
- Quantitative Biology and Bioinformatics, VTT Technical Research Centre of Finland, Espoo, Finland
-
visa färre...
-
(creator_code:org_t)
- Elsevier, 2010
- 2010
- Engelska.
-
Ingår i: Journal of Pharmaceutical and Biomedical Analysis. - : Elsevier. - 0731-7085 .- 1873-264X. ; 51:1, s. 125-130
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- A novel method for investigating drug release in a dynamic manner from nanoparticles including, but not limited to, biodegradable poly(lactic acid) (PLA) is reported. The PLA nanoparticles were prepared by the nanoprecipitation method. Two poorly soluble drugs, beclomethasone dipropionate (BDP) and indomethacin, were encapsulated into PLA nanoparticles, and their dissolution from the nanoparticles were followed in a dynamic way. The on-line method comprised a short column (vessel) packed with the PLA nanoparticles, on-line connected to an analytical liquid chromatographic column via a multiport switching valve equipped with two loops. The system allowed monitoring of the drug release profiles in real time, and the conditions for the drug release could be precisely controlled and easily changed. The effects of solvent composition and temperature on the rate of dissolution of the drugs from the PLA nanoparticles were investigated. The system proved to be linear for the drugs tested over the concentration range 10-3000 ng (n = 6, R(2) = 0.999 and 0.997 for indomethacin and beclomethasone, respectively) and repeatable (RSD of peak areas <0.5%). The recoveries of the dissolution study were quantitative (120 and 103% for indomethacin and beclomethasone, respectively).
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Poly(lactic acid); Beclomethasone dipropionate; Indomethacin; Liquid chromatography; Dissolution; Drug release
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas