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Abstract
Ämnesord
Stäng
- The affinity of T-cell receptors (TCRs) for major histocompatibility complex molecules (MHCs) presenting cognate antigens likely determines whether T cells initiate immune responses, or not. There exist few measurements of two-dimensional (2D) TCR-MHC interactions, and the effect of auxiliary proteins on binding is unexplored. Here, Jurkat T-cells expressing the MHC molecule HLA-DQ8-glia-α1 and the ligand of an adhesion protein (rat CD2) were allowed to bind supported lipid bilayers (SLBs) presenting fluorescently-labelled L3-12 TCR and rat CD2, allowing measurements of binding unconfounded by cell signaling effects or co-receptor binding. The 2D Kd for L3-12 TCR binding to HLA-DQ8-glia-α1, 14±5 molecules/μm2, was only marginally influenced by including CD2 up to ∼200 bound molecules/μm2 but higher CD2 densities reduced the affinity up to 1.9-fold. Cell-SLB contact size increased steadily with ligand density without affecting binding for contacts up to ∼20% of total cell area but beyond this lamellipodia appeared, giving an apparent increase in bound receptors of up to 50%. Our findings show how parameters other than the specific protein-protein interaction can influence binding behavior at cell-cell contacts.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Affinity
- CD2
- Lamellipodia
- Major histocompatibility complex
- Protein binding
- T-cell receptor
- Affinity
- CD2
- Lamellipodia
- Major histocompatibility complex
- Protein binding
- T-cell receptor
Publikations- och innehållstyp
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- Av författaren/redakt...
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Junghans, Victor ...
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Chouliara, Manto
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Santos, Ana Mafa ...
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Hatherley, Debor ...
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Petersen, Jan
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Dam, Tommy
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visa fler...
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Svensson, Lena M ...
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Rossjohn, Jamie
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Davis, Simon J.
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Jönsson, Peter
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Immunologi inom ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Cellbiologi
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Journal of Cell ...
- Av lärosätet
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Örebro universitet
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Lunds universitet