Sökning: onr:"swepub:oai:DiVA.org:oru-87720" >
Development of a Mu...
Development of a Multivalent Kunjin Virus Reporter Virus-Like Particle System Inducing Seroconversion for Ebola and West Nile Virus Proteins in Mice
-
- Tran, Pham Tue Hung, 1991- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Inflammatory Response and Infection Susceptibility Centre (iRiSC)
-
- Asghar, Naveed, 1983- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Inflammatory Response and Infection Susceptibility Centre (iRiSC)
-
- Höglund, Urban (författare)
- Adlego Biomedical AB, Uppsala, Sweden
-
visa fler...
-
- Larsson, Olivia (författare)
- Adlego Biomedical AB, Uppsala, Sweden
-
- Haag, Lars (författare)
- Karolinska Institutet
-
- Mirazimi, Ali (författare)
- Karolinska Institutet
-
- Johansson, Magnus, 1969- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Inflammatory Response and Infection Susceptibility Centre (iRiSC)
-
- Melik, Wessam, 1973- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Inflammatory Response and Infection Susceptibility Centre (iRiSC)
-
visa färre...
-
(creator_code:org_t)
- 2020-11-29
- 2020
- Engelska.
-
Ingår i: Microorganisms. - Basel, Switzerland : MDPI. - 2076-2607. ; 8:12
- Relaterad länk:
-
https://doi.org/10.3...
-
visa fler...
-
https://oru.diva-por... (primary) (Raw object)
-
https://www.mdpi.com...
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Kunjin virus (KUNV) is an attenuated strain of the severe neurotropic West Nile virus (WNV). The virus has a single-strand positive-sense RNA genome that encodes a polyprotein. Following gene expression, the polyprotein is cleaved into structural proteins for viral packaging and nonstructural proteins for viral replication and expression. Removal of the structural genes generate subgenomic replicons that maintain replication capacity. Co-expression of these replicons with the viral structural genes produces reporter virus-like particles (RVPs) which infect cells in a single round. In this study, we aimed to develop a system to generate multivalent RVPs based on KUNV to elicit an immune response against different viruses. We selected the Ebola virus (EBOV) glycoprotein (GP) and the matrix protein (VP40) genes, as candidates to be delivered by KUNV RVPs. Initially, we enhanced the production of KUNV RVPs by generating a stable cell line expressing the KUNV packaging system comprising capsid, precursor membrane, and envelope. Transfection of the DNA-based KUNV replicon into this cell line resulted in an enhanced RVP production. The replicon was expressed in the stable cell line to produce the RVPs that allowed the delivery of EBOV GP and VP40 genes into other cells. Finally, we immunized BALB/cN mice with RVPs, resulting in seroconversion for EBOV GP, EBOV VP40, WNV nonstructural protein 1, and WNV E protein. Thus, our study shows that KUNV RVPs may function as a WNV vaccine candidate and RVPs can be used as a gene delivery system in the development of future EBOV vaccines.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Annan medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Other Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Nyckelord
- Reporter virus-like particles (RVPs)
- replicons
- Kunjin virus
- Ebola virus
- glycoprotein (GP)
- matrix protein (VP40)
- packaging system
- stable cell line
- seroconversion
- vaccines
- Molecular Biology
- Molekylärbiologi
- Biomedicin
- Biomedicine
- Molekylär cellbiologi
- Molecular Cellbiology
- Biomedicinsk laboratorievetenskap
- Biomedical Laboratory Science
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Tran, Pham Tue H ...
-
Asghar, Naveed, ...
-
Höglund, Urban
-
Larsson, Olivia
-
Haag, Lars
-
Mirazimi, Ali
-
visa fler...
-
Johansson, Magnu ...
-
Melik, Wessam, 1 ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Annan medicinsk ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Mikrobiologi ino ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Immunologi
- Artiklar i publikationen
-
Microorganisms
- Av lärosätet
-
Örebro universitet
-
Karolinska Institutet