Sökning: onr:"swepub:oai:DiVA.org:oru-93506" >
Estradiol Alters th...
Estradiol Alters the Virulence Traits of Uropathogenic Escherichia coli
-
- Engelsöy, Ulrik, 1991- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper
-
- Svensson, Maria, 1980- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Research and Education
-
- Demirel, Isak, 1987- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,iRiSC-Inflammatory Response and Infection Susceptibility Centre
-
(creator_code:org_t)
- 2021-07-20
- 2021
- Engelska.
-
Ingår i: Frontiers in Microbiology. - : Frontiers Media S.A.. - 1664-302X. ; 12
- Relaterad länk:
-
https://doi.org/10.3...
-
visa fler...
-
https://www.frontier...
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- Uropathogenic Escherichia coli (UPEC) is the most common bacteria to cause urinary tract infection (UTI). Postmenopausal women have an increased risk of recurrent UTI. This is partly explained by estrogenic effects on host defenses against UTI. Current research is mostly focused on how UPEC affects host factors, but not so much is known about how host factors like hormones affect UPEC virulence. The aim of the present study was to investigate the impact of estradiol exposure on the virulence of UPEC. We found that a postmenopausal concentration of estradiol increased CFT073 growth and biofilm formation, but not the premenopausal concentrations. Real-time qPCR showed that estradiol altered the expression of genes associated with the iron acquisition system and metabolic pathways in CFT073. We also found that estradiol in a dose-dependent manner increased the expression of fimH and papC adhesins and increased colonization and invasion of bladder epithelial cells. The premenopausal concentration of estradiol also suppressed cytokine release from bladder epithelial cells. Additionally, we also showed using a Caenorhabditis elegans killing assay that estradiol increased the survival of CFT073-infected C. elegans worms. Taken together, our findings show that estradiol has the ability to alter the virulence traits of UPEC.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- Cross-kingdom interaction
- estradiol
- growth
- uropathogenic Escherichia coli
- virulence
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas