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Qualitative and qua...
Qualitative and quantitative comparison of allergen component-specific to birch and grass analyzed by ImmunoCAP assay and Euroline immunoblot test
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- Berge, M. (författare)
- Department of Otolaryngology, Örebro University Hospital, Örebro, Sweden
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- Bertilsson, L. (författare)
- Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden
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- Hultgren, Olof, 1970- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department Clinical Immunology and Transfusion Medicine
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- Hugosson, Svante, 1956- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department Clinical Immunology and Transfusion Medicine
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- Saber, Amanj, 1972- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department Clinical Immunology and Transfusion Medicine
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(creator_code:org_t)
- Edizioni Edra, 2023
- 2023
- Engelska.
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Ingår i: European annals of allergy and clinical immunology. - : Edizioni Edra. - 1764-1489. ; 55:2, s. 68-77
- Relaterad länk:
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https://doi.org/10.2...
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- Background: In the diagnostic work up of allergy, determining allergen component-specific immunoglobulin E (IgE) is important for diagnosis, prognosis and choice of treatment.Objective: The purpose of this study was to evaluate the performance of the immunoblotting assay (Euroline) in detection of IgE antibodies against timothy grass and birch pollen allergen components compared to fluorescent enzyme assay (ImmunoCAP, Phadia 250).Methods: A total of 128 serum samples from patients allergic to timothy grass and birch pollen were analysed. The levels of IgE antibodies to timothy grass and birch pollen were measured using Euroline DPA-Dx pollen 1 and ImmunoCAP assay. The two methods were then compared on binary (positive vs negative), semi-quantitative (IgE classes) and quantitative (concentration) levels. The two methods were also compared to results from skin prick testing.Results: The Euroline method showed a positive percentage agreement of 93% and negative percentage agreement of 94% with an overall accuracy of 94% when compared to ImmunoCAP. Kappa analysis showed moderate strength of agreement between the methods in determining IgE classes for 7/11 components tested. All components showed a positive correlation when analysed using Spearman's rank correlation.Conclusions: Overall, we found that there is good correlation between the Euroline and ImmunoCAP methods in measuring IgE sensitization.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Nyckelord
- Allergen-specific IgE
- Euroline
- ImmunoCAP
- pollen
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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