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The influence of hy...
The influence of hyaluronan on the structure of a DPPC-bilayer under high pressures
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- Zander, Thomas (författare)
- Helmholtz-Zentrum Geesthacht, Germany
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- Wieland, D. C. Florian (författare)
- Helmholtz-Zentrum Geesthacht, Germany
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- Raj, Akanksha (författare)
- KTH,Yt- och korrosionsvetenskap
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- Wang, Min (författare)
- KTH,Yt- och korrosionsvetenskap
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- Nowak, Benedikt (författare)
- Technical University of Dortmund, Germany
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- Krywka, Christina (författare)
- Helmholtz-Zentrum Geesthacht, Germany
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- Dédinaité, Andra (författare)
- KTH,RISE,Life Science,KTH Royal Institute of Technology, Sweden,Tillämpad fysikalisk kemi,SP Tech Res Inst Sweden
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- Claesson, Per Martin (författare)
- KTH,RISE,Material och ytteknik,KTH Royal Institute of Technology, Sweden,Yt- och korrosionsvetenskap,SP Tech Res Inst Sweden
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- Garamus, Vasil M. (författare)
- Helmholtz-Zentrum Geesthacht, Germany
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- Schreyer, Andreas (författare)
- Helmholtz-Zentrum Geesthacht, Germany
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- Willumeit-Römer, Regine (författare)
- Helmholtz-Zentrum Geesthacht, Germany
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(creator_code:org_t)
- Elsevier, 2016
- 2016
- Engelska.
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Ingår i: Colloids and Surfaces B. - : Elsevier. - 0927-7765 .- 1873-4367. ; 142, s. 230-238
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The superior lubrication properties of synovial joints have inspired many studies aiming at uncovering the molecular mechanisms which give rise to low friction and wear. However, the mechanisms are not fully understood yet, and, in particular, it has not been elucidated how the biolubricants present at the interface of cartilage respond to high pressures, which arise during high loads of joints. In this study we utilize a simple model system composed of two biomolecules that have been implied as being important for joint lubrication. It consists of a solid supported dipalmitoylphosphatidylcholin (DPPC) bilayer, which was formed via vesicles fusion on a flat Si wafer, and the anionic polysaccharide hyaluronan (HA). We first characterized the structure of the HA layer that adsorbed to the DPPC bilayers at ambient pressure and different temperatures using X-ray reflectivity (XRR) measurements. Next, XRR was utilized to evaluate the response of the system to high hydrostatic pressures, up to 2 kbar (200 MPa), at three different temperatures. By means of fluorescence microscopy images the distribution of DPPC and HA on the surface was visualized. Our data suggest that HA adsorbs to the headgroup region that is oriented towards the water side of the supported bilayer. Phase transitions of the bilayer in response to temperature and pressure changes were also observed in presence and absence of HA. Our results reveal a higher stability against high hydrostatic pressures for DPPC/HA composite layers compared to that of the DPPC bilayer in absence of HA.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Nyckelord
- Lubrication
- Synovial joint
- Vesicle fusion
- Supported DPPC bilayer
- Hyaluronan
- High pressure
- X-ray reflectivity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Zander, Thomas
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Wieland, D. C. F ...
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Raj, Akanksha
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Wang, Min
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Nowak, Benedikt
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Krywka, Christin ...
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visa fler...
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Dédinaité, Andra
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Claesson, Per Ma ...
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Garamus, Vasil M ...
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Schreyer, Andrea ...
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Willumeit-Römer, ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Fysikalisk kemi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Materialkemi
- Artiklar i publikationen
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Colloids and Sur ...
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