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Graphene oxide is d...
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Mukherjee, Sourav P.Karolinska Institutet, Sweden
(författare)
Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic
- Artikel/kapitelEngelska2018
Förlag, utgivningsår, omfång ...
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2018
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Royal Society of Chemistry (RSC),2018
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:ri-33247
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https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-33247URI
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https://doi.org/10.1039/c7nr03552gDOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-222253URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:137538348URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Funding details: University of Manchester; Funding details: R01ES019304, NIEHS, National Institute of Environmental Health Sciences; Funding details: 604391, EC, European Commission; Funding details: 696656, EC, European Commission; Funding details: TEM, Työ- ja Elinkeinoministeriö; Funding details: AFM-Téléthon; Funding text: This work was supported by the European Commission through the GRAPHENE Flagship Project (grant agreements no. 604391, and 696656) and the National Institute of Environmental Health Sciences (R01ES019304). We thank the University of Manchester Bioimaging Facility for the use of the TEM and AFM instruments for the characterization of GO.
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QC 20180205
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Neutrophils were previously shown to digest oxidized carbon nanotubes through a myeloperoxidase (MPO)-dependent mechanism, and graphene oxide (GO) was found to undergo degradation when incubated with purified MPO, but there are no studies to date showing degradation of GO by neutrophils. Here we produced endotoxin-free GO by a modified Hummers' method and asked whether primary human neutrophils stimulated to produce neutrophil extracellular traps or activated to undergo degranulation are capable of digesting GO. Biodegradation was assessed using a range of techniques including Raman spectroscopy, transmission electron microscopy, atomic force microscopy, and mass spectrometry. GO sheets of differing lateral dimensions were effectively degraded by neutrophils. As the degradation products could have toxicological implications, we also evaluated the impact of degraded GO on the bronchial epithelial cell line BEAS-2B. MPO-degraded GO was found to be non-cytotoxic and did not elicit any DNA damage. Taken together, these studies have shown that neutrophils can digest GO and that the biodegraded GO is non-toxic for human lung cells.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Gliga, Anda R.Karolinska Institutet
(författare)
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Lazzaretto, BeatriceKarolinska Institutet
(författare)
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Brandner, BirgitKarolinska Institutet,RISE,Yta, process och formulering
(författare)
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Fielden, MatthewKTH,Tillämpad fysik(Swepub:kth)u156snz9
(författare)
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Vogt, CarmenKTH,Biomedicinsk fysik och röntgenfysik(Swepub:kth)u1iann4b
(författare)
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Newman, LeonUniversity of Manchester, UK
(författare)
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Rodrigues, Artur F.University of Manchester, UK
(författare)
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Shao, WentingUniversity of Pittsburgh, USA
(författare)
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Fournier, Philip M.University of Pittsburgh, USA
(författare)
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Toprak, Muhammet SadakaKTH,Biomedicinsk fysik och röntgenfysik(Swepub:kth)u1u3m5a2
(författare)
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Star, AlexanderUniversity of Pittsburgh, USA
(författare)
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Kostarelos, KostasUniversity of Manchester, UK
(författare)
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Bhattacharya, KunalKarolinska Institutet, Sweden
(författare)
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Fadeel, BengtKarolinska Institutet, Sweden
(författare)
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Karolinska InstitutetKarolinska Institutet, Sweden
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Nanoscale: Royal Society of Chemistry (RSC)10:3, s. 1180-11882040-33642040-3372
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Mukherjee, Soura ...
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Gliga, Anda R.
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Lazzaretto, Beat ...
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Brandner, Birgit
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Fielden, Matthew
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Vogt, Carmen
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Newman, Leon
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Rodrigues, Artur ...
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Shao, Wenting
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Fournier, Philip ...
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Toprak, Muhammet ...
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Star, Alexander
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Kostarelos, Kost ...
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Bhattacharya, Ku ...
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Fadeel, Bengt
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