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Viral detection and...
Viral detection and quantification in a digital droplet microfluidic lab-in-a-fiber device
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- Parker, Helen E. (författare)
- KTH,Laserfysik
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- Sengupta, Sanghamitra (författare)
- KTH,Laserfysik
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- Harish, Achar Vasant (författare)
- KTH,Laserfysik
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- Soares, Ruben R. G. (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi
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- Jönsson, Håkan, PhD, 1979- (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Margulis, Walter (författare)
- KTH,RISE,Smart hårdvara,KTH Royal Institute of Technology, Sweden,Laserfysik,Res Inst Sweden RISE, Stockholm, Sweden.
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- Russom, Aman, Prof. 1976- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi
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- Laurell, Fredrik, professor, 1957- (författare)
- KTH,Laserfysik
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(creator_code:org_t)
- SPIE, 2021
- 2021
- Engelska.
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 9781510643802
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- In this work, we present the design and fabrication of a fiber device that performs digital droplet microfluidics for molecular diagnostics. A variety of fibers and capillaries were used to build three connected modules dedicated to droplet generation, incubation, and fluorescence detection which enables a uniaxial arrangement. This is in contrast to the traditional 2-dimensional lab-on-a-chip architecture. We characterize our fiber device using a fluorescein dilution series. Our observed detection limit is on the order of 10 nM fluorescein. We demonstrate our all-fiber device for the fluorescence readout after loop-mediated isothermal amplification (LAMP) of synthetic SARS-CoV-2. Our results suggest that this fiber device can successfully distinguish between positive and negative samples in molecular diagnostics. We propose that our fiber device offers benefits over microfluidic chip techniques such as easier optical integration, much simpler sample loading, and faster diagnosis with high specificity and sensitivity. Keywords: All-fiber device, microfluidics, optofluidics, loop-mediated isothermal amplification (LAMP), molecular diagnostics, SARS-CoV2.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Digital microfluidics
- Diseases
- Drops
- Fluorescence
- Isotherms
- Microstructure
- Chip architecture
- Droplet generation
- Droplet microfluidics
- Fluorescence detection
- Loop mediated isothermal amplifications
- Microfluidic chip
- Molecular diagnostics
- Optical integration
- Optical fibers
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Parker, Helen E.
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Sengupta, Sangha ...
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Harish, Achar Va ...
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Soares, Ruben R. ...
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Jönsson, Håkan, ...
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Margulis, Walter
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visa fler...
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Russom, Aman, Pr ...
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Laurell, Fredrik ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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Proceedings of S ...
- Micro-structured ...
- Av lärosätet
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RISE
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Kungliga Tekniska Högskolan