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Production of Exopo...
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Chambi, DiegoUmeå universitet,Kemiska institutionen,Instituto de Investigacion y Desarrollo de Procesos Quimicos, Chemical Engineering, Faculty of Engineering, Universidad Mayor de San Andres, P.O. Box 12958, La Paz, Bolivia; Viceministerio de Producción Industrial a Mediana y Gran Escala, Ministerio de Desarrollo Productivo y Economía Plural, P.O. Box 12958, La Paz, Bolivia,Umeå University, Sweden; Universidad Mayor de San Andrés, Bolivia; Ministerio de Desarrollo Productivo y Economía Plural, Bolivia
(författare)
Production of Exopolysaccharides by Cultivation of Halotolerant Bacillus atrophaeus BU4 in Glucose- and Xylose-Based Synthetic Media and in Hydrolysates of Quinoa Stalks
- Artikel/kapitelEngelska2022
Förlag, utgivningsår, omfång ...
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2022-02-14
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MDPI AG,2022
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:ri-58591
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https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-58591URI
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https://doi.org/10.3390/fermentation8020079DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-192785URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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This research was funded by the Swedish Research Council (2016-05822) and the Bio4Energyresearch environment (www.bio4energy.se; accessed on 20 December 2021).
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A halotolerant, exopolysaccharide-producing bacterium isolated from the Salar de Uyuni salt flat in Bolivia was identified as Bacillus atrophaeus using next-generation sequencing. Comparisons indicate that the genome most likely (p-value: 0.0024) belongs to a subspecies previously not represented in the database. The growth of the bacterial strain and its ability to produce exopolysaccharides (EPS) in synthetic media with glucose or xylose as carbon sources, and in hydrolysates of quinoa stalks, was investigated. The strain grew well in all synthetic media, but the growth in glucose was better than that in xylose. Sugar consumption was better when initial concentrations were low. The growth was good in enzymatically produced cellulosic hydrolysates but was inhibited in hemicellulosic hydrolysates produced using hydrothermal pretreatment. The EPS yields were up to 0.064 g/g on initial glucose and 0.047 g/g on initial xylose, and was higher in media with relatively low sugar concentrations. The EPS was isolated and purified by a sequential procedure including centrifugation, cold ethanol precipitation, trichloroacetic acid treatment, dialysis, and freeze-drying. Glucose and mannose were the main sugars identified in hydrolyzed EPS. The EPS was characterized by size-exclusion chromatography, Fourier-transform infrared (FTIR) spectroscopy, heteronuclear single-quantum coherence nuclear magnetic resonance (HSQC NMR) spectroscopy, scanning electron microscopy, X-ray diffraction, and thermogravimetric analysis. No major differences were elucidated between EPS resulting from cultivations in glucose- or-xylose-based synthetic media, while some divergences with regard to molecular-weight averages and FTIR and HSQC NMR spectra were detected for EPS from hydrolysate-based media.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Lundqvist, Jenny,1975-Umeå universitet,Kemiska institutionen,Umeå University, Sweden(Swepub:umu)jeylst96
(författare)
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Nygren, ErikRISE,Jordbruk och livsmedel,Department of Agrifood and Bioscience, RISE Research Institutes of Sweden AB, P.O. Box 857, Borås, Sweden(Swepub:ri)erik.nygren@ri.se
(författare)
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Romero-Soto, LuisInstituto de Investigacion y Desarrollo de Procesos Quimicos, Chemical Engineering, Faculty of Engineering, Universidad Mayor de San Andres, P.O. Box 12958, La Paz, Bolivia
(författare)
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Marin, KatherineUmeå universitet,Kemiska institutionen,Instituto de Investigacion y Desarrollo de Procesos Quimicos, Chemical Engineering, Faculty of Engineering, Universidad Mayor de San Andres, P.O. Box 12958, La Paz, Bolivia,Umeå University, Sweden; Universidad Mayor de San Andrés, Bolivia
(författare)
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Gorzsás, AndrásUmeå universitet,Kemiska institutionen,Umeå University, Sweden(Swepub:umu)goan0002
(författare)
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Hedenström, Mattias,1976-Umeå universitet,Kemiska institutionen,Umeå University, Sweden(Swepub:umu)mashem96
(författare)
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Carlborg, Markus,1986-Umeå universitet,Institutionen för tillämpad fysik och elektronik,Umeå University, Sweden(Swepub:umu)maca0028
(författare)
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Broström, MarkusUmeå universitet,Institutionen för tillämpad fysik och elektronik,Umeå University, Sweden(Swepub:umu)maskon97
(författare)
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Sundman, OlaUmeå universitet,Kemiska institutionen,Umeå University, Sweden(Swepub:umu)olasun02
(författare)
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Carrasco, CristhianInstituto de Investigacion y Desarrollo de Procesos Quimicos, Chemical Engineering, Faculty of Engineering, Universidad Mayor de San Andres, P.O. Box 12958, La Paz, Bolivia
(författare)
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Jönsson, Leif J.Umeå universitet,Kemiska institutionen,Umeå University, Sweden(Swepub:umu)leja0012
(författare)
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Martín, CarlosUmeå universitet,Kemiska institutionen,Department of Biotechnology, Inland Norway University of Applied Sciences, Hamar, Norway,Umeå University, Sweden; Inland Norway University of Applied Sciences, Norway(Swepub:umu)cama0153
(författare)
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Umeå universitetKemiska institutionen
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Fermentation: MDPI AG8:22311-5637
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Chambi, Diego
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Lundqvist, Jenny ...
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Nygren, Erik
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Romero-Soto, Lui ...
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Marin, Katherine
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Gorzsás, András
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Hedenström, Matt ...
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Carlborg, Markus ...
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Broström, Markus
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Sundman, Ola
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Carrasco, Cristh ...
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Jönsson, Leif J.
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Martín, Carlos
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