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Sökning: onr:"swepub:oai:DiVA.org:ri-73289" > Deciphering the rol...

  • Ranji, ParmidaUniversity of Gothenburg, Sweden (författare)

Deciphering the role of FUS::DDIT3 expression and tumor microenvironment in myxoid liposarcoma development

  • Artikel/kapitelEngelska2024

Förlag, utgivningsår, omfång ...

  • BioMed Central Ltd,2024
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:ri-73289
  • https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-73289URI
  • https://doi.org/10.1186/s12967-024-05211-wDOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Open access funding provided by University of Gothenburg. This research was funded by Assar Gabrielssons Research Foundation; Johan Jansson Foundation for Cancer Research; Region Västra Götaland, Sweden; Swedish Cancer Society (2022-2080 and 2022-2214); Swedish Childhood Cancer Foundation (2022-0030); Swedish Research Council (2021-01008 and 2019-01273); the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement (965065 and 965580) and Sweden's Innovation Agency (2017-03737); the Sjöberg Foundation and Wilhelm and Martina Lundgren Foundation for Scientific Research.
  • Background: Myxoid liposarcoma (MLS) displays a distinctive tumor microenvironment and is characterized by the FUS::DDIT3 fusion oncogene, however, the precise functional contributions of these two elements remain enigmatic in tumor development. Methods: To study the cell-free microenvironment in MLS, we developed an experimental model system based on decellularized patient-derived xenograft tumors. We characterized the cell-free scaffold using mass spectrometry. Subsequently, scaffolds were repopulated using sarcoma cells with or without FUS::DDIT3 expression that were analyzed with histology and RNA sequencing. Results: Characterization of cell-free MLS scaffolds revealed intact structure and a large variation of protein types remaining after decellularization. We demonstrated an optimal culture time of 3 weeks and showed that FUS::DDIT3 expression decreased cell proliferation and scaffold invasiveness. The cell-free MLS microenvironment and FUS::DDIT3 expression both induced biological processes related to cell-to-cell and cell-to-extracellular matrix interactions, as well as chromatin remodeling, immune response, and metabolism. Data indicated that FUS::DDIT3 expression more than the microenvironment determined the pre-adipocytic phenotype that is typical for MLS. Conclusions: Our experimental approach opens new means to study the tumor microenvironment in detail and our findings suggest that FUS::DDIT3-expressing tumor cells can create their own extracellular niche.

Ämnesord och genrebeteckningar

  • MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
  • MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
  • MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
  • MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
  • Animals; Cell Line
  • Tumor; Cell Proliferation; Cell-Free System; Extracellular Matrix; Gene Expression Regulation
  • Neoplastic; Humans; Liposarcoma
  • Myxoid; Mice; Oncogene Proteins
  • Fusion; RNA-Binding Protein FUS; Tissue Scaffolds; Tumor Microenvironment; eosin; growth arrest and DNA damage inducible protein 153; hematoxylin; HLA antigen; major histocompatibility antigen class 2; RNA binding protein FUS; FUS protein
  • human; FUS-DDIT3 fusion protein
  • human; oncogene fusion protein; RNA binding protein FUS; adipogenesis; animal cell; animal experiment; animal model; antigen presentation; Article; cell adhesion; cell culture; cell cycle; cell growth; cell infiltration; cell interaction; cell invasion; cell migration; cell proliferation; chromatin assembly and disassembly; controlled study; decellularization; down regulation; endocytosis; experimental model; extracellular matrix; female; functional enrichment analysis; gene expression; gene expression profiling; gene fusion; genetic transcription; glycolysis; histology; HT-1080 cell line; human; human cell; immune response; in vivo study; liquid chromatography-mass spectrometry; mass spectrometry; metabolism; mouse; myxosarcoma; nonhuman; nucleotide metabolism; oncogene; phenotype; protein expression; proteomics; RNA extraction; RNA sequencing; RNA synthesis; sarcoma cell; single cell RNA seq; tumor cell; tumor microenvironment; tumor xenograft; upregulation; animal; cell free system; chemistry; gene expression regulation; genetics; pathology; tumor cell line

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Jonasson, EmmaUniversity of Gothenburg, Sweden (författare)
  • Andersson, LisaUniversity of Gothenburg, Sweden (författare)
  • Filges, StefanUniversity of Gothenburg, Sweden (författare)
  • Luna Santamaría, ManuelUniversity of Gothenburg, Sweden (författare)
  • Vannas, ChristofferUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden (författare)
  • Dolatabadi, SoheilaUniversity of Gothenburg, Sweden (författare)
  • Gustafsson, AnnaUniversity of Gothenburg, Sweden (författare)
  • Myklebost, OlaOslo University Hospital, Norway; University of Bergen, Norway (författare)
  • Håkansson, JoakimRISE,Metodik för produktframtagning,University of Gothenburg, Sweden(Swepub:ri)JoakimHa@ri.se (författare)
  • Fagman, HenrikUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden (författare)
  • Landberg, GöranUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden (författare)
  • Åman, PierreUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden (författare)
  • Ståhlberg, AndersUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden (författare)
  • University of Gothenburg, SwedenUniversity of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Journal of Translational Medicine: BioMed Central Ltd221479-5876

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