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Activator-binding d...
Activator-binding domains of the SWI/SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo
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- Ferreira, Monica E. (författare)
- Södertörns högskola,Molekylärbiologi,Karolinska instiutet
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- Prochasson, Philippe (författare)
- Karolinska Institutet
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- Berndt, Kurt D. (författare)
- Karolinska Institutet,Södertörns högskola,Kemi,Karolinska institutet
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- Workman, Jerry L. (författare)
- Stowers Institute for Medical Research, Kansas City, MO, USA
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- Wright, Anthony P. H. (författare)
- Södertörns högskola,Molekylärbiologi,Karolinska institutet
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(creator_code:org_t)
- Wiley, 2009
- 2009
- Engelska.
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Ingår i: The FEBS Journal. - : Wiley. - 1742-464X .- 1742-4658. ; 276:9, s. 2557-2565
- Relaterad länk:
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https://febs.onlinel...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Interaction between acidic activation domains and the activator-binding domains of Swi1 and Snf5 of the yeast SWI/SNF chromatin remodeling complex has previously been characterized in vitro. Although deletion of both activator-binding domains leads to phenotypes that differ from the wild-type, their relative importance for SWI/SNF recruitment to target genes has not been investigated. In the present study, we used chromatin immunoprecipitation assays to investigate the individual and collective importance of the activator-binding domains for SWI/SNF recruitment to genes within the GAL regulon in vivo. We also investigated the consequences of defective SWI/SNF recruitment for target gene activation. We demonstrate that deletion of both activator-binding domains essentially abolishes galactose-induced SWI/SNF recruitment and causes a reduction in transcriptional activation similar in magnitude to that associated with a complete loss of SWI/SNF activity. The activator-binding domains in Swi1 and Snf5 make approximately equal contributions to the recruitment of SWI/SNF to each of the genes studied. The requirement for SWI/SNF recruitment correlates with GAL genes that are highly and rapidly induced by galactose.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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