Selection of cefoxitin-resistant Bacteroides thetaiotaomicron mutants and mechanisms involved in beta-lactam resistance

The beta-lactam antibiotics are the most widely used of all the groups of antimicrobials, but beta-lactam resistance is increasingly common among members of the Bacteroides fragilis group. Three major mechanisms are involved in beta-lactam resistance, and they act together in certain instances. In the present study, 2 resistant mutants (238m and 1186m) of Bacteroides thetaiotaomicron, obtained from clinical isolates (238 and 1186) by selection with increasing concentrations of cefoxitin, showed decreased susceptibilities to cefoxitin and other beta-lactam antibiotics. Alterations in both penicillin-binding proteins (PBPs) and outer-membrane proteins (OMPs) were observed in the mutants in comparison with their parent strains. The similar alteration in OMPs was also observed in clinical isolates. In conclusion, the beta-lactam-resistant mutants of B. thetaiotaomicron with deficiency in both PBPs and OMPs can be selected for by exposure to cefoxitin, and several mechanisms are involved in the beta-lactam resistance in the strains investigated.

Edlund, CharlottaKarolinska Institutet,Södertörns högskola,Avdelning Naturvetenskap(Swepub:sh)SHCAED (författare)
Nord, C E (författare)
Hedberg, M (författare)
Karolinska InstitutetAvdelning Naturvetenskap (creator_code:org_t)

Ingår i:Clinical Infectious Diseases: Oxford University Press (OUP)35, s. S47-S531058-48381537-6591

Av författaren/redakt...: Fang, Hong; Edlund, Charlott ...; Nord, C E; Hedberg, M

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