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Bacterial chitinase...
Bacterial chitinase with phytopathogen control capacity from suppressive soil revealed by functional metagenomics
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- Hjort, Karin (författare)
- Uppsala universitet,Södertörns högskola,Biologi,Uppsala University,Sara Sjöling,Institutionen för medicinsk biokemi och mikrobiologi
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- Presti, Ilaria (författare)
- University of Insubria, Varese, Italy,Sara Sjöling
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- Elväng, Annelie (författare)
- Södertörns högskola,Biologi,Sara Sjöling
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- Marinelli, Flavia (författare)
- University of Insubria, Varese, Italy
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- Sjöling, Sara (författare)
- Södertörns högskola,Biologi,Sara Sjöling
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(creator_code:org_t)
- 2013-10-13
- 2014
- Engelska.
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Ingår i: Applied Microbiology and Biotechnology. - : Springer Science and Business Media LLC. - 0175-7598 .- 1432-0614. ; 98:6, s. 2819-2828
- Relaterad länk:
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https://doi.org/10.1...
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https://sh.diva-port... (primary) (Raw object)
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https://link.springe...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Plant disease caused by fungal pathogens results in vast crop damage globally. Microbial communities of soil that is suppressive to fungal crop disease provide a source for the identification of novel enzymes functioning as bioshields against plant pathogens. In this study, we targeted chitin-degrading enzymes of the uncultured bacterial community through a functional metagenomics approach, using a fosmid library of a suppressive soil metagenome. We identified a novel bacterial chitinase, Chi18H8, with antifungal activity against several important crop pathogens. Sequence analyses show that the chi18H8 gene encodes a 425-amino acid protein of 46 kDa with an N-terminal signal peptide, a catalytic domain with the conserved active site F175DGIDIDWE183, and a chitinase insertion domain. Chi18H8 was expressed (pGEX-6P-3 vector) in Escherichia coli and purified. Enzyme characterization shows that Chi18H8 has a prevalent chitobiosidase activity with a maximum activity at 35 °C at pH lower than 6, suggesting a role as exochitinase on native chitin. To our knowledge, Chi18H8 is the first chitinase isolated from a metagenome library obtained in pure form and which has the potential to be used as a candidate agent for controlling fungal crop diseases. Furthermore, Chi18H8 may also answer to the demand for novel chitin-degrading enzymes for a broad range of other industrial processes and medical purposes.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
Nyckelord
- metagenomic library
- chitinase
- terminal restriction fragment length polymorphism (T-RFLP)
- Streptomycetes
- suppressive soil
- Östersjö- och Östeuropaforskning
- Baltic and East European studies
- Environmental Studies
- Miljövetenskapliga studier
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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