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Locomotor pattern i...
Locomotor pattern in the adult zebrafish spinal cord in vitro
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Gabriel, J. P. (författare)
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Mahmood, R. (författare)
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Walter, A. M. (författare)
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Kyriakatos, A. (författare)
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- Hauptmann, Giselbert (författare)
- Karolinska Institutet,Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institute
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Calabrese, R. L. (författare)
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- El Manira, A. (författare)
- Karolinska Institutet
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(creator_code:org_t)
- American Physiological Society, 2008
- 2008
- Engelska.
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Ingår i: Journal of Neurophysiology. - : American Physiological Society. - 0022-3077 .- 1522-1598. ; 99:1, s. 37-48
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The zebrafish is an attractive model system for studying the function of the spinal locomotor network by combining electrophysiological, imaging, and genetic approaches. Thus far, most studies have been focusing on embryonic and larval stages. In this study we have developed an in vitro preparation of the isolated spinal cord from adult zebrafish in which locomotor activity can be induced while the activity of single neurons can be monitored using whole cell recording techniques. Application of NMDA elicited rhythmic locomotor activity that was monitored by recording from muscles or ventral roots in semi-intact or isolated spinal cord preparations, respectively. This rhythmic activity displayed a left-right alternation and a rostrocaudal delay. Blockade of glycinergic synaptic transmission by strychnine switched the alternating activity into synchronous bursting in the left and right sides as well as along the rostrocaudal axis. Whole cell recordings from motoneurons showed that they receive phasic synaptic inputs that were correlated with the locomotor activity recorded in ventral roots. This newly developed in vitro preparation of the adult zebrafish spinal cord will allow examination of the organization of the spinal locomotor network in an adult system to complement studies in zebrafish larvae and new born rodents.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Nyckelord
- n methyl dextro aspartic acid
- strychnine
- animal tissue
- article
- controlled study
- correlation analysis
- locomotion
- motoneuron
- muscle
- nerve cell
- neurotransmission
- nonhuman
- priority journal
- spinal cord
- synapse
- ventral root
- whole cell
- zebra fish
- Action Potentials
- Animals
- Biological Clocks
- Excitatory Amino Acid Agonists
- Functional Laterality
- Glycine
- Models
- Biological
- Motor Neurons
- Nerve Net
- Neural Pathways
- Patch-Clamp Techniques
- Periodicity
- Spinal Nerve Roots
- Synaptic Transmission
- Zebrafish
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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