Sökning: onr:"swepub:oai:DiVA.org:sh-22889" >
Single particle ref...
Single particle refinement in electron crystallography : A pilot study
-
- Koeck, Philip J. B. (författare)
- Karolinska Institutet,KTH,Södertörns högskola,Institutionen för livsvetenskaper,KTH / Karoliska institutet,Strukturell bioteknik
-
- Purhonen, P. (författare)
- Karolinska Institutet
-
- Alvang, Ronny (författare)
- KTH,Skolan för teknik och hälsa (STH)
-
visa fler...
-
- Grundberg, Björn (författare)
- KTH,Strukturell bioteknik
-
- Hebert, Hans (författare)
- Karolinska Institutet,KTH,Strukturell bioteknik
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
-
Ingår i: Journal of Structural Biology. - : Elsevier BV. - 1047-8477 .- 1095-8657. ; 160:3, s. 344-352
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Electron crystallography can be used to determine the structures of membrane proteins at near-atomic resolution in some cases. However, most electron crystallography projects remain at a resolution around 10 Å. This might be partly due to lack of flatness of many two-dimensional crystals. We have investigated this problem and suggest single particle processing of locally averaged unit cells to improve the quality and possibly the resolution of three-dimensional maps. Applying this method to the secondary transporter melibiose permease we have calculated a three-dimensional map that is clearer and easier to interpret than the map derived using purely electron-crystallographic methods.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Crystal distortion
- Electron crystallography
- Membrane proteins
- Single particle reconstruction
- melibiose
- membrane protein
- permease
- article
- calculation
- crystal structure
- crystallography
- elementary particle
- measurement
- pilot study
- priority journal
- protein structure
- structure analysis
- three dimensional imaging
- Computer Simulation
- Crystallization
- Escherichia coli Proteins
- Fourier Analysis
- Imaging
- Three-Dimensional
- Microscopy
- Electron
- Transmission
- Models
- Molecular
- Pilot Projects
- Protein Conformation
- Symporters
- Biology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas