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Reduction of ubiqui...
Reduction of ubiquinone by lipoamide dehydrogenase : An antioxidant regenerating pathway
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- Xia, Ling (författare)
- Södertörns högskola,Avdelning Naturvetenskap,Karolinska Institute
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- Björnstedt, M. (författare)
- Karolinska Institutet
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- Nordman, Tomas (författare)
- Södertörns högskola,Avdelning Naturvetenskap,Karolinska Institute
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- Eriksson, L. C. (författare)
- Karolinska Institutet
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Olsson, J. M. (författare)
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(creator_code:org_t)
- 2002-04-05
- 2001
- Engelska.
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Ingår i: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 268:5, s. 1486-1490
- Relaterad länk:
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https://febs.onlinel...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Lipoamide dehydrogenase belongs to a family of pyridine nucleotide disulfide oxidoreductases and is ubiquitous in aerobic organisms. This enzyme also reduces ubiquinone (the only endogenously synthesized lipid-soluble antioxidant) to ubiquinol, the form in which it functions as an antioxidant. The reduction of ubiquinone was linear with time and exhibited turnover numbers of 5 and 1.2 min-1 in the presence and absence of zinc, respectively. The reaction was stimulated by zinc and cadmium but not by the other divalent ions tested. The zinc/cadmium-dependent stimulation of the reaction increased rapidly and linearly up to a concentration of 0.1 mM and was even further increased at 0.5 mM. At pH 6, the activity was three times higher than at physiological pH. Alteration of the NADPH : NADP+ ratio revealed that the reaction is inhibited by higher concentrations of the oxidized cofactors. FAD reduced ubiquinone in a dose-dependent manner at a considerably lower rate, suggesting that the reduction of ubiquinone by lipoamide dehydrogenase involves the FAD moiety of the enzyme.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Antioxidants
- Lipoamide dehydrogenase
- Oxidative stress
- Ubiquinone
- Zinc
- antioxidant
- cadmium
- dihydrolipoamide dehydrogenase
- nicotinamide adenine dinucleotide phosphate
- oxidoreductase
- reduced nicotinamide adenine dinucleotide phosphate
- article
- controlled study
- dose response
- high performance liquid chromatography
- priority journal
- reduction
- Animals
- Cations
- Divalent
- Chromatography
- High Pressure Liquid
- Coenzymes
- Flavin-Adenine Dinucleotide
- Heart
- Hydrogen-Ion Concentration
- Kinetics
- Lipid Peroxidation
- NAD
- NADP
- Oxidation-Reduction
- Swine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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