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Deep sequencing ana...
Deep sequencing analysis of tick-borne encephalitis virus from questing ticks at natural foci reveals similarities between quasispecies pools of the virus
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- Asghar, Naveed (författare)
- Örebro universitet,Södertörns högskola,Biologi,School of Natural Science, Technology & Environmental Studies, Södertörn University, Huddinge, Sweden; iRiSC – Inflammatory Response and Infection Susceptibility Centre, Faculty of Medicine and Health, Örebro University, Örebro, Sweden,Institutionen för medicinska vetenskaper,Sodertorn Univ, Sch Nat Sci Technol & Environm Studies, Huddinge, Sweden.;Orebro Univ, Sch Med Sci, Orebro, Sweden.;Orebro Univ, Fac Med & Hlth, iRiSC, Orebro, Sweden.
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- Pettersson, John H.-O. 1981- (författare)
- Uppsala universitet,Systematisk biologi,Norwegian Inst Publ Hlth, Dept Infect Dis Epidemiol & Modelling, Oslo, Norway.;Natl Vet Inst, Dept Microbiol, Uppsala, Sweden.
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- Dinnétz, Patrik (författare)
- Södertörns högskola,Miljövetenskap,School of Natural Science, Technology & Environmental Studies, Södertörn University, Huddinge, Sweden,Sodertorn Univ, Sch Nat Sci Technol & Environm Studies, Huddinge, Sweden.
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- Andreassen, Åshild (författare)
- Department of Virology, Division of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway,Norwegian Inst Publ Hlth, Div Infect Dis Control, Dept Virol, Oslo, Norway.
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- Johansson, Magnus (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,iRiSC – Inflammatory Response and Infection Susceptibility Centre,Orebro Univ, Sch Med Sci, Orebro, Sweden.;Orebro Univ, Fac Med & Hlth, iRiSC, Orebro, Sweden.
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(creator_code:org_t)
- The Microbiology Society, 2017
- 2017
- Engelska.
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Ingår i: Journal of General Virology. - : The Microbiology Society. - 0022-1317 .- 1465-2099. ; 98:3, s. 413-421
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Abstract
Ämnesord
Stäng
- Every year, tick-borne encephalitis virus (TBEV) causes severe central nervous system infection in 10 000 to 15 000 people in Europe and Asia. TBEV is maintained in the environment by an enzootic cycle that requires a tick vector and a vertebrate host, and the adaptation of TBEV to vertebrate and invertebrate environments is essential for TBEV persistence in nature. This adaptation is facilitated by the error-prone nature of the virus's RNA-dependent RNA polymerase, which generates genetically distinct virus variants called quasispecies. TBEV shows a focal geographical distribution pattern where each focus represents a TBEV hotspot. Here, we sequenced and characterized two TBEV genomes, JP-296 and JP-554, from questing Ixodes ricinus ticks at a TBEV focus in central Sweden. Phylogenetic analysis showed geographical clustering among the newly sequenced strains and three previously sequenced Scandinavian strains, Toro-2003, Saringe-2009 and Mandal-2009, which originated from the same ancestor. Among these five Scandinavian TBEV strains, only Mandal-2009 showed a large deletion within the 3' non-coding region (NCR), similar to the highly virulent TBEV strain Hypr. Deep sequencing of JP-296, JP-554 and Mandal-2009 revealed significantly high quasispecies diversity for JP-296 and JP-554, with intact 3' NCRs, compared to the low diversity in Mandal-2009, with a truncated 3' NCR. Single-nucleotide polymorphism analysis showed that 40% of the single-nucleotide polymorphisms were common between quasispecies populations of JP-296 and JP-554, indicating a putative mechanism for how TBEV persists and is maintained within its natural foci.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- :Ixodes ricinus; natural foci; non-coding region; quasispecies; Scandinavia; tick-borne encephalitis virus
- Environmental Studies
- Miljövetenskapliga studier
- Östersjö- och Östeuropaforskning
- Baltic and East European studies
- Microbiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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