Sökning: onr:"swepub:oai:DiVA.org:sh-31884" >
Structural analysis...
Structural analysis of lipopolysaccharides from Haemophilus influenzae serotype f - Structural diversity observed in three strains
-
- Yildirim, Håkan H. (författare)
- Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet
-
- Hood, D W (författare)
- University of Oxford, John Radcliffe Hostpital, Oxford, UK
-
- Moxon, E R (författare)
- University of Oxford, John Radcliffe Hostpital, Oxford, UK
-
visa fler...
-
- Schweda, Elke K. H. (författare)
- Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet
-
visa färre...
-
(creator_code:org_t)
- Wiley, 2003
- 2003
- Engelska.
-
Ingår i: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 270:15, s. 3153-3167
- Relaterad länk:
-
https://febs.onlinel...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Structural elucidation of the lipopolysaccharide (LPS) from three serotype f Haemophilus influenzae clinical isolates RM6255, RM7290 and RM6252 has been achieved using NMR spectroscopy techniques and ESI-MS on O-deacylated LPS and core oligosaccharide material (OS) as well as ESI-MSn on permethylated dephosphorylated OS. This is the first study to report structural details on LPS from serotype f strains. We found that the LPSs of all strains were highly heterogeneous mixtures of glycoforms expressing the common H. influenzae structural element l-alpha-d-Hepp -(1-->2)-[P Etn-->6]-l-alpha-d-Hepp -(1-->3)-[beta-d-Glcp -(1-->4)]-l-alpha-d-Hepp -(1-->5)-[PP Etn-->4]-alpha-Kdo-(2-->6)-lipid A with variable length of OS chains linked to each of the heptoses. The terminal heptose (HepIII) in RM6255 is substituted at the O-3 position by a beta-d-Glcp residue whereas HepIII in strains RM7290 and RM6252 is substituted at O-2 by the globoside unit (alpha-d-Galp -(1-->4)-beta-d-Galp -(1-->4)-beta-d-Glc) or truncated versions thereof. The central heptose (HepII) is substituted by an alpha-d-Galp -(1-->4)-beta-d-Galp -(1-->4)-beta-d-Glcp -(1-->4)-alpha-d-Glcp unit in RM7290 and RM6252 or truncated versions thereof. Strain RM6255 does not express galactose in its LPS and only shows a cellobiose unit elongating from HepII (beta-d-Glcp -(1-->4)-alpha-d-Glcp ). ESI-MSn on dephosphorylated and permethylated OS provided information on the existence of additional minor isomeric glycoforms.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Haemophilus influenzae
- lipopolysaccharide
- NMR
- ESI-MS
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas