Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays

The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.

Hassan, A HPennsylvania State University, Pennsylvania, USA (författare)
Wallberg, Annika ESödertörns högskola,Avdelning Naturvetenskap,Karolinska Institutet (författare)
Steger, D JPennsylvania State University, Pennsylvania, USA (författare)
Cairns, B RUniversity of Utah School of Medicine, Salt Lake City, USA (författare)
Wright, Athony P HSödertörns högskola,Avdelning Naturvetenskap,Karolinska Institutet(Swepub:sh)SHAYWT (författare)
Workman, J LPennsylvania State University, Pennsylvania, USA (författare)
Pennsylvania State University, Pennsylvania, USAAvdelning Naturvetenskap (creator_code:org_t)

Av författaren/redakt...: Neely, K E; Hassan, A H; Wallberg, Annika ...; Steger, D J; Cairns, B R; Wright, Athony P ...; visa fler...; Workman, J L; visa färre...

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