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A peptide nucleic a...
A peptide nucleic acid–nuclear localization signal fusion that mediates nuclear transport of DNA
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- Brandén, Lars J. (författare)
- Karolinska Institutet, Sweden; Huddinge Hospital, Sweden
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- Mohamed, Abdalla J (författare)
- Södertörns högskola,Avdelning Naturvetenskap,Karolinska Institutet, Sweden
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- Smith, C. I. Edvard (författare)
- Karolinska Institutet,Karolinska Institutet, Sweden; Huddinge Hospital, Sweden
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(creator_code:org_t)
- Springer Science and Business Media LLC, 1999
- 1999
- Engelska.
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Ingår i: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 17:8, s. 784-787
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We have combined a peptide nucleic acid (PNA) with the SV40 core nuclear localization signal (NLS), to create a bifunctional PNA–NLS peptide. The PNA–NLS peptide increased the nuclear uptake of oligonucleotides and enhanced the transfection efficacy of plasmids. Gene expression from an enhanced green fluorescent protein plasmid and a lacZ plasmid was preserved when hybridized to PNA–NLS. In combination with the transfection agent polyethyleneimine, we have improved both the nuclear translocation of fluorescence-marked oligonucleotides, and the efficacy of plasmid transfection, up to eightfold. The technique obviates the use of cumbersome coupling procedures of the vector due to DNA–PNA duplex formation or displacement of the antisense plasmid DNA strand by a PNA molecule.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- Nuclear localization signal
- Oligonucleotide
- Peptide nucleic acid
- Plasmid
- Transfection
- Translocation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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