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Glutathione transfe...
Glutathione transferases immobilized on nanoporous alumina : Flow system kinetics, screening, and stability
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- Kjellander, Marcus (författare)
- Uppsala universitet,Biokemi
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- Mazari, Aslam M. A. (författare)
- Stockholms universitet,Institutionen för neurokemi,Department of Neurochemistry, Stockholm University, SE-10691, Sweden
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- Boman, Mats (författare)
- Uppsala universitet,Oorganisk kemi
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- Mannervik, Bengt (författare)
- Uppsala universitet,Stockholms universitet,Institutionen för neurokemi,Uppsala University, Sweden,Biokemi
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- Johansson, Gunnar (författare)
- Uppsala universitet,Biokemi
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(creator_code:org_t)
- Elsevier BV, 2014
- 2014
- Engelska.
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Ingår i: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 446, s. 59-63
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The previously uncharacterized Drosophila melanogaster Epsilon-class glutathione transferases E6 and E7 were immobilized on nanoporous alumina. The nanoporous anodized alumina membranes were derivatized with 3-aminopropyl-triethoxysilane, and the amino groups were activated with carbonyldiimidazole to allow coupling of the enzymes via c-amino groups. Kinetic analyses of the immobilized enzymes were carried out in a circulating flow system using CDNB (1-chloro-2,4-dinitrobenzene) as substrate, followed by specificity screening with alternative substrates. A good correlation was observed between the substrate screening data for immobilized enzyme and corresponding data for the enzyme in solution. A limited kinetic study was also carried out on immobilized human GST S1-1 (also known as hematopoietic prostaglandin D synthase). The stability of the immobilized enzymes was virtually identical to that of enzymes in solution, and no leakage of enzyme from the matrix could be observed.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Immobilization
- Glutathione transferase
- Kinetics
- Screening
- Enzyme reactor
- Drosophila
- neurokemi med molekylär neurobiologi
- Neurochemistry with Molecular Neurobiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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