Glutathione transferases immobilized on nanoporous alumina: Flow system kinetics, screening, and stability

Glutathione transferases immobilized on nanoporous alumina : Flow system kinetics, screening, and stability

AuthorCount:5;
The previously uncharacterized Drosophila melanogaster Epsilon-class glutathione transferases E6 and E7 were immobilized on nanoporous alumina. The nanoporous anodized alumina membranes were derivatized with 3-aminopropyl-triethoxysilane, and the amino groups were activated with carbonyldiimidazole to allow coupling of the enzymes via c-amino groups. Kinetic analyses of the immobilized enzymes were carried out in a circulating flow system using CDNB (1-chloro-2,4-dinitrobenzene) as substrate, followed by specificity screening with alternative substrates. A good correlation was observed between the substrate screening data for immobilized enzyme and corresponding data for the enzyme in solution. A limited kinetic study was also carried out on immobilized human GST S1-1 (also known as hematopoietic prostaglandin D synthase). The stability of the immobilized enzymes was virtually identical to that of enzymes in solution, and no leakage of enzyme from the matrix could be observed.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
Immobilization
Glutathione transferase
Kinetics
Screening
Enzyme reactor
Drosophila
neurokemi med molekylär neurobiologi
Neurochemistry with Molecular Neurobiology

Mazari, Aslam M. A.Stockholms universitet,Institutionen för neurokemi,Department of Neurochemistry, Stockholm University, SE-10691, Sweden(Swepub:su)muas4460 (författare)
Boman, MatsUppsala universitet,Oorganisk kemi(Swepub:uu)matsboma (författare)
Mannervik, BengtUppsala universitet,Stockholms universitet,Institutionen för neurokemi,Uppsala University, Sweden,Biokemi(Swepub:uu)bengmann (författare)
Johansson, GunnarUppsala universitet,Biokemi(Swepub:uu)gunnjoha (författare)
Uppsala universitetBiokemi (creator_code:org_t)

Av författaren/redakt...: Kjellander, Marc ...; Mazari, Aslam M. ...; Boman, Mats; Mannervik, Bengt; Johansson, Gunna ...

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