Sökning: onr:"swepub:oai:DiVA.org:su-118282" > Ultrafiltration wit...
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000 | 04417naa a2200697 4500 | |
001 | oai:DiVA.org:su-118282 | |
003 | SwePub | |
008 | 150615s2015 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:umu-104379 | |
009 | oai:prod.swepub.kib.ki.se:131270848 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1182822 URI |
024 | 7 | a https://doi.org/10.1016/j.nano.2015.01.0032 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1043792 URI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1312708482 URI |
040 | a (SwePub)sud (SwePub)umud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Nordin, Joel Z.u Karolinska Institutet4 aut |
245 | 1 0 | a Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties |
264 | 1 | b Elsevier BV,c 2015 |
338 | a print2 rdacarrier | |
520 | a Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) and differential ultracentrifugation (UC). A significantly higher EV yield resulted from UF-LC as compared to UC, without affecting vesicle protein composition. Importantly, we provide novel evidence that, in contrast to UC-purified EVs, the biophysical properties of UF-LC-purified EVs are preserved, leading toadifferent in vivo biodistribution, with less accumulation in lungs. Finally, we show that UF-LC is scalable and adaptable for EV isolation from complex media types such as stem cell media, which is of huge significance for future clinical applications involving EVs. | |
650 | 7 | a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng |
653 | a Biophysical properties | |
653 | a extracellular vesicles | |
653 | a size-exclusion liquid chromatography | |
653 | a ultracentrifugation | |
653 | a ultrafiltration | |
700 | 1 | a Lee, Yiu Karolinska Institutet4 aut |
700 | 1 | a Vader, Pieter4 aut |
700 | 1 | a Mäger, Imre4 aut |
700 | 1 | a Johansson, Henrik J.u Karolinska Institutet4 aut |
700 | 1 | a Heusermann, Wolf4 aut |
700 | 1 | a Wiklander, Oscar P. B.u Karolinska Institutet4 aut |
700 | 1 | a Hällbrink, Mattiasu Stockholms universitet,Institutionen för neurokemi4 aut0 (Swepub:su)mhall |
700 | 1 | a Seow, Yiqi4 aut |
700 | 1 | a Bultema, Jarred J.4 aut |
700 | 1 | a Gilthorpe, Jonathanu Umeå universitet,Umeå centrum för molekylär medicin (UCMM)4 aut0 (Swepub:umu)jogi0002 |
700 | 1 | a Davies, Tim4 aut |
700 | 1 | a Fairchild, Paul J.4 aut |
700 | 1 | a Gabrielsson, Susanneu Karolinska Institutet4 aut |
700 | 1 | a Meisner-Kober, Nicole C.4 aut |
700 | 1 | a Lehtio, Janneu Karolinska Institutet4 aut |
700 | 1 | a Smith, C. I. Edvardu Karolinska Institutet4 aut |
700 | 1 | a Wood, Matthew J. A.4 aut |
700 | 1 | a Andaloussi, Samir E. L.u Karolinska Institutet4 aut |
710 | 2 | a Karolinska Institutetb Institutionen för neurokemi4 org |
773 | 0 | t Nanomedicined : Elsevier BVg 11:4, s. 879-883q 11:4<879-883x 1549-9634x 1549-9642 |
856 | 4 | u https://doi.org/10.1016/j.nano.2015.01.003y Fulltext |
856 | 4 | u https://doi.org/10.1016/j.nano.2015.01.003 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-118282 |
856 | 4 8 | u https://doi.org/10.1016/j.nano.2015.01.003 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-104379 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:131270848 |
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