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Sökning: onr:"swepub:oai:DiVA.org:su-124913" > Peptide nanoparticl...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004270naa a2200469 4500
001oai:DiVA.org:su-124913
003SwePub
008160106s2015 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:130935549
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1249132 URI
0247 a https://doi.org/10.1089/nat.2014.05112 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1309355492 URI
040 a (SwePub)sud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Järver, Peteru Medical Research Council , Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 aut
2451 0a Peptide nanoparticle delivery of charge-neutral splice-switching morpholino oligonucleotides.
264 1b Mary Ann Liebert Inc,c 2015
338 a print2 rdacarrier
520 a Oligonucleotide analogs have provided novel therapeutics targeting various disorders. However, their poor cellular uptake remains a major obstacle for their clinical development. Negatively charged oligonucleotides, such as 2'-O-Methyl RNA and locked nucleic acids have in recent years been delivered successfully into cells through complex formation with cationic polymers, peptides, liposomes, or similar nanoparticle delivery systems. However, due to the lack of electrostatic interactions, this promising delivery method has been unsuccessful to date using charge-neutral oligonucleotide analogs. We show here that lipid-functionalized cell-penetrating peptides can be efficiently exploited for cellular transfection of the charge-neutral oligonucleotide analog phosphorodiamidate morpholino. The lipopeptides form complexes with splice-switching phosphorodiamidate morpholino oligonucleotide and can be delivered into clinically relevant cell lines that are otherwise difficult to transfect while retaining biological activity. To our knowledge, this is the first study to show delivery through complex formation of biologically active charge-neutral oligonucleotides by cationic peptides.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
7001 a Zaghloul, Eman Mu Karolinska Institutet4 aut
7001 a Arzumanov, Andrey Au Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 aut
7001 a Saleh, Amer Fu Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 aut
7001 a McClorey, Grahamu Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom4 aut
7001 a Hammond, Suzan Mu Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom4 aut
7001 a Hällbrink, Mattiasu Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital, Huddinge, Sweden4 aut0 (Swepub:su)mhall
7001 a Langel, Ülou Stockholms universitet,Institutionen för neurokemi4 aut0 (Swepub:su)ulo
7001 a Smith, C I Edvardu Karolinska Institutet4 aut
7001 a Wood, Matthew J Au Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom4 aut
7001 a Gait, Michael Ju Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 aut
7001 a El Andaloussi, Samiru Karolinska Institutet4 aut
7102 a Karolinska Institutetb Medical Research Council , Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 org
7730 t Nucleic Acid Therapeuticsd : Mary Ann Liebert Incg 25:2, s. 65-77q 25:2<65-77x 2159-3337x 2159-3345
8564 u https://doi.org/10.1089/nat.2014.0511y Fulltext
8564 u https://www.liebertpub.com/doi/pdf/10.1089/nat.2014.0511
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-124913
8564 8u https://doi.org/10.1089/nat.2014.0511
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:130935549

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