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Production of indiv...
Production of individualized V gene databases reveals high levels of immunoglobulin genetic diversity
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- Corcoran, Martin M. (författare)
- Karolinska Institutet
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- Phad, Ganesh E. (författare)
- Karolinska Institutet
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- Bernat, Nestor Vazquez (författare)
- Karolinska Institutet
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Stahl-Hennig, Christiane (författare)
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- Sumida, Noriyuki (författare)
- Karolinska Institutet
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- Persson, Mats A. A. (författare)
- Karolinska Institutet
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- Martin, Marcel (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
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- Hedestam, Gunilla B. Karlsson (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2016-12-20
- 2016
- Engelska.
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Ingår i: nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 7
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Comprehensive knowledge of immunoglobulin genetics is required to advance our understanding of B cell biology. Validated immunoglobulin variable (V) gene databases are close to completion only for human and mouse. We present a novel computational approach, IgDiscover, that identifies germline V genes from expressed repertoires to a specificity of 100%. IgDiscover uses a cluster identification process to produce candidate sequences that, once filtered, results in individualized germline V gene databases. IgDiscover was tested in multiple species, validated by genomic cloning and cross library comparisons and produces comprehensive gene databases even where limited genomic sequence is available. IgDiscover analysis of the allelic content of the Indian and Chinese-origin rhesus macaques reveals high levels of immunoglobulin gene diversity in this species. Further, we describe a novel human IGHV3-21 allele and confirm significant gene differences between Balb/c and C57BL6 mouse strains, demonstrating the power of IgDiscover as a germline V gene discovery tool.
Ämnesord
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
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