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Macrophage activati...
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Gallud, AudreyKarolinska Institutet
(författare)
Macrophage activation status determines the internalization of mesoporous silica particles of different sizes : Exploring the role of different pattern recognition receptors
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
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Elsevier BV,2017
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-141226
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-141226URI
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https://doi.org/10.1016/j.biomaterials.2016.12.029DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:135163359URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Mesoporous silica-based particles are promising candidates for biomedical applications. Here, we address the importance of macrophage activation status for internalization of AMS6 (approx. 200 nm in diameter) versus AMS8 (approx. 2 mu m) mesoporous silica particles and the role of different phagocytosis receptors for particle uptake. To this end, FITC-conjugated silica particles were used. AMS8 were found to be non-cytotoxic both for M-CSF-stimulated (anti-inflammatory) and GM-CSF-stimulated (pro-inflammatory) macrophages, whereas AMS6 exhibited cytotoxicity towards M-CSF-stimulated, but not GMCSF-stimulated macrophages; this toxicity was, however, mitigated in the presence of serum. AMS8 triggered the secretion of pro-inflammatory cytokines in M-CSF-activated cells. Class A scavenger receptor (SR-A) expression was noted in both M-CSF and GM-CSF-stimulated macrophages, although the expression was higher in the former case, and gene silencing of SR-A resulted in a decreased uptake of AMS6 in the absence of serum. GM-CSF-stimulated macrophages expressed higher levels of the mannose receptor CD206 compared to M-CSF-stimulated cells, and uptake of AMS6, but not AMS8, was reduced following the downregulation of CD206 in GM-CSF-stimulated cells; particle uptake was also suppressed by mannan, a competitive ligand. These studies demonstrate that macrophage activation status is an important determinant of particle uptake and provide evidence for a role of different macrophage receptors for cell uptake of silica particles.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bondarenko, Olesja
(författare)
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Feliu, NeusKarolinska Institutet
(författare)
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Kupferschmidt, NataliaStockholms universitet,Institutionen för material- och miljökemi (MMK)(Swepub:su)naku4385
(författare)
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Atluri, Rambabu
(författare)
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Garcia-Bennett, Alfonso
(författare)
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Fadeel, BengtKarolinska Institutet
(författare)
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Karolinska InstitutetInstitutionen för material- och miljökemi (MMK)
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biomaterials: Elsevier BV121, s. 28-400142-96121878-5905
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