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Enhanced susceptibi...
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Gutzkow, K. B.
(författare)
Enhanced susceptibility of obese mice to glycidamide-induced sperm chromatin damage without increased oxidative stress
- Artikel/kapitelEngelska2016
Förlag, utgivningsår, omfång ...
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2016-08-30
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Wiley,2016
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-144569
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-144569URI
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https://doi.org/10.1111/andr.12233DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Diet-induced obesity is known to impair male reproduction and may aggravate the male reproductive toxicity of the food contaminant acrylamide. Exposure of male mice to acrylamide induces paternally mediated pre- and post-implantation losses because of spermatozoal toxicity and these effects are potentiated in mice fed a high-fat diet. Glycidamide - an acrylamide metabolite - is the primary mediator of reproductive effects in males. The mechanisms causing the interaction between diet and acrylamide are not clear. However, diet-induced obesity is associated with oxidative stress in male reproductive tissues which might contribute to increased germ cell susceptibility. In this study, we investigated whether a moderate diet-induced obesity regimen could interfere with glycidamide-induced spermatozoal toxicity and increase oxidative stress. For this purpose, sperm chromatin integrity, oxidised DNA and protein levels, transcript levels of oxidative stress responsive genes and glycidamide-induced DNA and haemoglobin adducts were analysed in samples from male mice exposed to a high-fat diet for 6 weeks in combination with a single glycidamide exposure 7 days prior to sacrifice. We found that glycidamide-induced sperm DNA fragmentation was markedly higher in obese than in lean mice. However, the levels of oxidised DNA and/or protein in blood, liver and testicular tissue was lower in obese than in lean mice. Accompanying the reduced level of oxidised macromolecules, the transcript levels of several oxidative stress-related genes were altered in the liver and testis from obese mice suggesting induction of an antioxidant response in these animals. The haemoglobin-glycidamide adduct levels were higher in obese than in lean animals, whereas obesity did not seem to increase the level of glycidamide-induced DNA adducts. These findings show that a moderate diet-induced obesity regimen may potentiate glycidamide-induced sperm cells toxicity and suggest that the increase in glycidamide-induced sperm toxicity observed in obese mice does not depend on overt oxidative stress.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Duale, N.
(författare)
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Danielsen, T.
(författare)
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von Stedingk, HansStockholms universitet,Institutionen för miljövetenskap och analytisk kemi
(författare)
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Shahzadi, S.
(författare)
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Instanes, C.
(författare)
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Olsen, A. -K.
(författare)
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Steffensen, I. -L.
(författare)
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Hofer, T.
(författare)
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Törnqvist, MargaretaStockholms universitet,Institutionen för miljövetenskap och analytisk kemi(Swepub:su)mt
(författare)
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Brunborg, G.
(författare)
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Lindeman, B.
(författare)
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Stockholms universitetInstitutionen för miljövetenskap och analytisk kemi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Andrology: Wiley4:6, s. 1092-11142047-29192047-2927
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Till lärosätets databas
- Av författaren/redakt...
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Gutzkow, K. B.
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Duale, N.
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Danielsen, T.
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von Stedingk, Ha ...
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Shahzadi, S.
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Instanes, C.
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visa fler...
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Olsen, A. -K.
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Steffensen, I. - ...
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Hofer, T.
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Törnqvist, Marga ...
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Brunborg, G.
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Lindeman, B.
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Reproduktionsmed ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Artiklar i publikationen
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Andrology
- Av lärosätet
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Stockholms universitet