Sökning: onr:"swepub:oai:DiVA.org:su-15054" >
c-Jun supports ribo...
c-Jun supports ribosomal RNA processing and nucleolar localization of RNA helicase DDX21
-
Holmström, Tim H. (författare)
-
Mialon, Antoine (författare)
-
Kallio, Marko (författare)
-
visa fler...
-
Nymalm, Yvonne (författare)
-
Mannermaa, Leni (författare)
-
- Holm, Tina (författare)
- Stockholms universitet,Institutionen för neurokemi
-
- Johansson, Henrik (författare)
- Stockholms universitet,Institutionen för neurokemi
-
Black, Elizabeth (författare)
-
Gillespie, David (författare)
-
Salminen, Tiina A. (författare)
-
- Langel, Ülo (författare)
- Stockholms universitet,Institutionen för neurokemi
-
Valdez, Benigno C. (författare)
-
Westermarck, Jukka (författare)
-
visa färre...
-
(creator_code:org_t)
- 2008
- 2008
- Engelska.
-
Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 283:11, s. 7046-7053
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The molecular mechanisms by which the AP-1 transcription factor c-Jun exerts its biological functions are not clearly understood. In addition to its well established role in transcriptional regulation of gene expression, several reports have suggested that c-Jun may also regulate cell behavior by non-transcriptional mechanisms. Here, we report that small interfering RNA-mediated depletion of c-Jun from mammalian cells results in inhibition of 28 S and 18 S rRNA accumulation. Moreover, we show that c-Jun depletion results in partial translocation of RNA helicase DDX21, implicated in rRNA processing, from the nucleolus to the nucleoplasm. We demonstrate that DDX21 translocation is rescued by exogenous c-Jun expression and that c-Jun depletion inhibits rRNA binding of DDX21. Furthermore, the direct interaction between c-Jun and DDX21 regulates nucleolar localization of DDX21. These results demonstrate that in addition to its transcriptional effects, c-Jun regulates rRNA processing and nucleolar compartmentalization of the rRNA processing protein DDX21. Thus, our results demonstrate a nucleolar mechanism through which c-Jun can regulate cell behavior. Moreover, these results suggest that the phenotypes observed previously in c-Jun-depleted mouse models and cell lines could be partly due to the effects of c-Jun on rRNA processing.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Holmström, Tim H ...
-
Mialon, Antoine
-
Kallio, Marko
-
Nymalm, Yvonne
-
Mannermaa, Leni
-
Holm, Tina
-
visa fler...
-
Johansson, Henri ...
-
Black, Elizabeth
-
Gillespie, David
-
Salminen, Tiina ...
-
Langel, Ülo
-
Valdez, Benigno ...
-
Westermarck, Juk ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Journal of Biolo ...
- Av lärosätet
-
Stockholms universitet