Sökning: onr:"swepub:oai:DiVA.org:su-167374" >
Padlock Probe-Based...
Padlock Probe-Based Nucleic Acid Amplification Tests : Point-of-care Diagnostics of Infectious Diseases
-
- Ciftci, Sibel, 1987- (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Mats Nilsson
-
- Nilsson, Mats, PhD, Prof. (preses)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Klingström, Jonas, PhD, Prof. (opponent)
- Karolinska Institute
-
(creator_code:org_t)
- ISBN 9789177975762
- Department of Biochemistry and Biophysics, Stockholm University, 2019
- Engelska.
- Relaterad länk:
-
https://su.diva-port... (Preview)
-
visa fler...
-
https://su.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Recent advancements in molecular biology and biotechnology have pushed the field of molecular diagnostics much further to benefit the society towards smart access for rapid and simplified health care. In this context, point-of-care (PoC) technologies that bring the inventions in diagnostics closer to bedside settings draw attention. This becomes all the more relevant in the case of infectious diseases which pose the major burden, in terms of mortality and economic loss, especially for third world developing countries with resource-limited settings (RLS). Moreover, emerging and re-emerging viruses, known for their rapid mutation rates, demand huge attention in terms of timely diagnosis and the need for effective treatments. Hence, appropriate and accurate tests to detect the pathogens with enhanced sensitivity and specificity would be needed to bridge the gap between bioanalytics and clinics.This research work is an attempt to combine the tools and techniques required for the development of such efficient PoC technologies to combat infectious diseases. Among available nucleic acid-based amplification tests, padlock probing and isothermal rolling circle amplification are used to benefit from the advantages they offer for diagnostic applications, in terms of specificity, multiplexability, single molecule detection, high throughput, compatibility with various read-out platforms and inexpensive digital quantification.In the first paper, simultaneous detection of RNA and DNA forms of adenovirus is shown to study the spatio-temporal expression patterns in both lytic and persistent infections. In situ quantification of viral DNA as well as transcripts with single cell resolution has been achieved. In the second paper, novel probe design strategy has been presented for the development of molecular assays to detect hypervariable RNA viruses. This approach becomes helpful in targeting rapidly evolving viruses by using mutation-tolerant probes for RCA. Third paper demonstrates simple RCA for rapid detection of Ebola virus in clinical samples, followed by a multiplexed detection with other re-emerging tropical viruses, namely Zika and Dengue. This study also includes a simple easy-to-operate pump-free membrane enrichment read-out, combined together with microscopy for digital quantification of the products. In the fourth paper, near point-of-care glucose sensor-based RCP detection has been proposed for Ebola virus detection. All these attempts clearly bring RCA closer to PoC settings for molecular diagnostics of virus infections.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Nucleic Acid Amplification
- Isothermal Amplification Methods
- Padlock Probes
- Rolling Circle Amplification
- Molecular Diagnostics
- Infectious Disease Diagnostics
- Virus
- Point-of-Care
- Biochemistry
- biokemi
Publikations- och innehållstyp
- vet (ämneskategori)
- dok (ämneskategori)
Hitta via bibliotek
Till lärosätets databas