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High throughput bar...
High throughput barcoding method for genome-scale phasing
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- Redin, David (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Frick, Tobias (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Aghelpasand, Hooman (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Käller, Max (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
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- Borgström, Erik (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
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- Olsen, Remi-Andre (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Box 1031, S-17121 Solna, Sweden.
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- Ahmadian, Afshin (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2019-12-02
- 2019
- Engelska.
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The future of human genomics is one that seeks to resolve the entirety of genetic variation through sequencing. The prospect of utilizing genomics for medical purposes require cost-efficient and accurate base calling, long-range haplotyping capability, and reliable calling of structural variants. Short-read sequencing has lead the development towards such a future but has struggled to meet the latter two of these needs. To address this limitation, we developed a technology that preserves the molecular origin of short sequencing reads, with an insignificant increase to sequencing costs. We demonstrate a novel library preparation method for high throughput barcoding of short reads where millions of random barcodes can be used to reconstruct megabase-scale phase blocks.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Medicinsk bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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